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M. Ohtsu et al.-1 #"/DG$48(:[email protected] àň ƅã 1 ǢĶ½ àƱ 1,2 Ǣİû Ì 1,2,3 1 ÅÀùàèàèǏ ŚèũŴŮ 464-8602 ēŨŦÅÀùýºŰ¹Ɔŝ 2 JSTz ERATO İût=gjx^FOhxN?F[ 464-8602 ēŨŦÅÀùýºŰ¹Ɔŝ 3 ÅÀùàè [tyOfA{lY<gśÊ§åũŴė 464-8601 ēŨŦÅÀùýºŰ¹Ɔŝ In planta deep-imaging of plant - plant-parasitic nematode interaction using cutting edge imaging methods Mina Ohtsu 1 , Daisuke Kurihara 1,2 , Tetsuya Higashiyama 1,2,3 1 Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8402, Japan 2 Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan 3 Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan Key words: plant-parasitic nematode, in planta deep imaging, two-photon excitation microscopy, plant-microbe interaction DOI: 10.24480/bsj-review.8a1.00109 IH ČśŔ&Ĕij%2ǢîĹŔ¤ǂ&žƃ!'Ǟ2$ݸƮ5&|"Ǣ ĹŔ&ĸ%ïś5ĹŔïśĐƁƔ`JgQyVr>ƨõ5üàżƊ1ǢMO[QyVr> ƨõ5ÃƊ&ćĖğ365 ( Ó 1) 63&ƁƔ%2ƨõ6ćĖ65žƃ'Ǣ ĸ&¤ǂ%ćĖ65/ǢÞǂ3ơò5"'Ǖþ%ǓǢ6,!&ũŴ!'%¨œ9 ŜơòƗ86Ǣ&ćĕ1ćĖoC^Pn9Ƨż%Śƣ5/%'ǢĔijž ƃ&ĪNjŢǢŵNjŢ$Ē×ĎƝÁĿ!5$3ǢĹŔżƊ&ćŖ&ƾ%25&ø Ĝ1ĤǢ,µƮ1ƕ9ǀ5ļ$¤ÕиÃŔ&çÕ$#%24ǢĹŔ&Ōǂ9ơò5& 'Ǖþ%ÑǓ!5ƴāǢĹŔžƃ%īƿ¸ƷĨ¸ƦƓ ClearSee 1 TOMEI &NJš%24ǢĹ ŔŌǂ=o{NyG'àÿ%ƻł5 (Kurihara et al. 2015, Hasegawa et al. 2016) 3%ǢĹŔ& Ōǂơò9Ɨ/%ǢŊƜĐ1Ōǂ¬ƽĐ"ś=o{NyG%ƿŕč9ĞǢ2 åµƮǚČLjʼnŜ65Įű!'ǢīĦLJ&ĹŔŌǂ=o{NyGęƘ9ǝǢĹŔï

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In planta deep-imaging of plant - plant-parasitic nematode interaction

using cutting edge imaging methods Mina Ohtsu1, Daisuke Kurihara1,2, Tetsuya Higashiyama1,2,3

1Graduate School of Science, Nagoya University!, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8402, Japan

2Higashiyama Live-Holonics Project, ERATO, JST, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8602, Japan

3Institute of Transformative Bio-Molecules (ITbM), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan

Key words: plant-parasitic nematode, in planta deep imaging, two-photon excitation microscopy,

plant-microbe interaction DOI: 10.24480/bsj-review.8a1.00109

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