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Transcript of { Bt cotton Bethany Zumwalde. What is Bt cotton? How is it produced? Advantages & limitations Bt...
![Page 1: { Bt cotton Bethany Zumwalde. What is Bt cotton? How is it produced? Advantages & limitations Bt cotton study Inheritance of an insecticidal.](https://reader035.fdocuments.net/reader035/viewer/2022062304/56649f1c5503460f94c31cda/html5/thumbnails/1.jpg)
{
Bt cotton
Bethany Zumwalde
![Page 2: { Bt cotton Bethany Zumwalde. What is Bt cotton? How is it produced? Advantages & limitations Bt cotton study Inheritance of an insecticidal.](https://reader035.fdocuments.net/reader035/viewer/2022062304/56649f1c5503460f94c31cda/html5/thumbnails/2.jpg)
What is Bt cotton? How is it produced?
Advantages & limitations
Bt cotton study Inheritance of an insecticidal
Gene (cry1Ab) in Genetically Modified Cotton
Bt controversy
Outline
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Genetically modified to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis.
Toxins are crystalline proteins (Cry-proteins) that target specific pests.
What is Bt cotton?
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How is it produced?
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Advantages & Limitations
Advantages
High insect specificity Control crop damage
and disease vectors Nontoxic to non-target
species Biodegradable Reduction of other
insecticides 94.5 million kg
(19.4%) from 1996 to 2005 for cotton
Yield increases
Limitations
Susceptible to resistance
High seed cost
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Case Study: Inheritance of an Insecticidal Gene (cry1Ab) in Genetically Modified Cotton1
(Ghazanfar et al. 2012)
Using the Cry1Ab gene to create 6 crosses (2 transgenic lines & 2 non-transgenic lines)
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Methods
(Ghazanfar et al. 2012)
PCR Southern blot Western blot Lab biotoxicity assay
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Results
(Ghazanfar et al. 2012)
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Highest heritability between crosses of transgenic and non-transgenic plants
Resistance was also highest in these crosses
The creation of new transgenic lines of Bt cotton is necessary to combat insect resistance
Conclusions
http://www.pakissan.com/english/advisory/biotechnology/images/bt.cotton.asia01.jpg
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To Bt or not to Bt?
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Questions?