MMP-2MMP-9MMP-2MMP-9-2

MMP-2MMP-9MMP-2MMP-9

X20000 Da

200866PDB

1 2 3 42D-NMRX-RAY

5656 7

PDB,SWISS-PROT

SWISS-MODEL SWISS-MODEL1993http://swissmodel.expasy.org//SWISS-MODEL.html

First Approach mode(25%Email

MMP-2MMP-9

Paper electrophorisis Cellulose Acetate electrophoresis Agar Gel electrophoresis Polyacrylamide Gel-electrophoresis SDS-SDS-PAGE,-

(acrylamideAcr)N,N-(N,N -methylene-bisacylamide,Bis) N,N,N,N-(N,N,N,N-tetram-ethyl ethylenedia mineTEMED)(ammonium persulfate (NH4)2S2O3AP) (polyacrylamide gel electrophoresisPAGE)N,N-

pHAcr10-6g

SDSPAGESDS-PAGE1967shapiroSDSSodium Dodecyl SulfateSDSSDS- SDS

(extracellular matrixECM)ECM(basement membranesBM)(interstitial connective tissue)ECM12I

ECM(plasminogen activator, PA)(metalproteinase, MMP)IV

MMPsMMPs 1MMP-1MMP-5MMP-8MMP-13, , 2MMP-2MMP-9,, 3MMP-3MMP-7MMP-10MMP-11(FN)(LN)

MMP-2A 72kDaMMP-9B92kDaMMPsneutrophil gelatinase associated lipocalinNGAL)1993KjeldsenMMP-9MMP-9MMP-9MMP-9MMP-2

N 310C N 310NGAL

MMP-2Pre: Pro: Zn: ZnII: IIH: Hemopexin

MMP-9525

NGALMMP-9MMP-9NGALpcDNA3 SHEEC pcDNA-NGAL (-) SHEEC MMP-9 MMP-2pcDNA-NGAL () SHEEC MMP-9 MMP-2NGAL SHEEC MMP-9 MMP-2 Mmarker1. 2. pcDNA33. pcDNA-NGAL ( + )4. pcDNA-NGAL ( - )

MMP-2MMP-9ZymographyMMP-2MMP-9SDS-SDS-PAGE0.1MMP-2MMP-9MMP-2MMP-9

: 2SDS ;: 10TEMED5ml3min4TEMED;

10 l; 4 100V2h; 11h 23724h 30.1%30min5 45

MMP-2MMP-9

MMP-2MMP-9MMP-2MMP-9