John Bleby – Sysmex UK

XN WPC Channel

How it works and the UK experience

Agenda

01

02 What is the WPC Channel?

04

03 How does it work?

XN in the UK and Ireland

Case studies and Performance

Agenda

01

XN in the UK and Ireland

XN Installations in the UK & Ireland

4

UK Market Situation

5

250 XN Series at 83 sites

(25 XN-20) 241 XE Series

(+80 XT)

UK XN App Sales

6

250 XN Analysers

90 Ret Licences

42 PLT-F Licences

34 BF Licences

25 WPC

What is the WPC Channel?

XN WPC Channel

The XN WPC channel is an extended

differential counting and flagging system.

Composed of additional:

• Hardware

• Software

• Reagents

Act as a team

• 9

» Maximum sensitivity

» Preclassification of clear cases

» Increased specificity

» Reduction of false positives

» Final classification reactive and malignant

XN WPC Channel

10

Hardware

The WPC application comes as standard

on the XN 20 unit, but is unavailable on

the XN 10. There is no ability to upgrade

an XN-10 to XN-20.

Reagents

The WPC channel uses two reagents. A

Lysercell surfactant reagent to gain

access to the cell and a Flurocell to stain.

XN WPC Channel

11

White Precursor Cell (WPC) screening identifies abnormal white blood cells

through the detection of an abnormal lipid composition in the cell membranes,

and is thus able to separate them from normal WBC.

The trigger for this analysis is an abnormal finding in the initial CBC+DIFF

analysis and a special rule set for reflex testing.

How does it work?

XN WDF and WPC

• Automated reflex of WPC following CBC/Diff analysis means that

there is no staff interaction required in order to further investigate.

• The WPC testing is triggered following the production of the

Blast/Abn Lymphs? Flag from the WDF channel.

• These results are interpreted together to get the best possible

picture of what the abnormal cell population is composed of.

13

Working together

14

Fl

Vol.

SSC

SSC

WDF [lipid membrane & RNA content]

WPC [Lipid membrane & DNA content]

L

M

N E

L M

N E

L

M N E

L

M N E

L

L

Fl

Vol.

SSC

SSC

XN Flagging Strategy

16

17

Case Studies and Performance

Case studies

19

Patient history: Lymphocytosis in a 53-year old woman.

Case studies

20

Diagnosis:

B-cell chronic lymphatic leukaemia (B-CLL)

Population of abnormal

Lymphocytes in SSC/SFL

WPC Channel

Case studies

Patient history: 54-year old man was admitted to the

hospital with a high fever.

21

Case studies

22

Diagnosis: Acute myeloid leukaemia (AML) under chemotherapy: AML with

abnormal marrow eosinophils

Population of Blasts in WDF/WPC Channel

Case studies

23

Patient history: a patient with a known disease, undergoing treatment.

Case studies

24

Diagnosis:

A 63-year old male patient suffering from mantle cell lymphoma had undergone

chemotherapy and his blood was examined to monitor the effect of the

treatment.

Population of abnormal lymphocytes in WDF/WPC Channel

XN WDF vs XE-5000

25

Jones AS, Tailor H, Liesner R, Machin SJ, Briggs CJ (2014).

The value of the white precursor cell channel (WPC) on the Sysmex XN-1000 analyser in a specialist

paediatric hospital. J Clin Pathol 68(2): 161

XN WDF+WPC vs XE-5000

26

Munich Großhadern Studies

27 27

Accepted in: Clinical Chemistry and Laboratory Medicine (CCLM, Feb.2015)

Any Questions?