Post on 05-Nov-2020
Welcome
National Reference Centre (NRC)
Mieke Reyniers
National Plant Protection Organization (NPPO-NL)The Netherlands
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NRC: part of the plant health system in the Netherlands
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Importance of good infrastructure
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• Naktuinbouw: the Netherlands Inspection Service for Horticulture• BKD: the Flower Bulb Inspection Service• NAK: the Dutch General Inspection Service• KCB: the Quality Control Bureau
Plant health system in the Netherlands: government
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National Reference Centre (NRC)
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2500 fellow workers
Headquartered in Utrecht
Inspection offices throughout the country
Wageningen: regional office + laboratories
Independent agency in the Ministry of Agriculture, Nature and Food Quality
Delivery agency for the Ministry of Health, Welfare and Sport.
Dutch-NPPO/NRC part of the Netherlands Food and Consumer Product Safety Authority (NVWA)
Meat supply chain and Food safety
Veterinary medicinalproducts
ExportImport
Product safety
Animal welfare
Hospitality industry and artisanal production
Alcohol andtobacco
Plant HealthNature
Fish, fish products and aquaculture
Animal testing
Crop protection Animal Health
Fertilizer
Animal feedEuropean subsidies Cross complianceGround-bound subsidies
Industrial productionAnimal by-productsMicrobioloy
NVWA covers 23 domains
Head
Mieke Reyniers
Plant DiseasesMaikel Aveskamp
- Bacteriology- Mycology- Virology
Plant PestsAnton van der Sommen
- Entomology- Nematology- Invasive plants- Centre for Monitoring vectors
Diagnostic methodsErik Souer
- Administration- Biosafety/-security- Molecular Biology- Greenhouses
National Reference Center – plant healthmore than a lab
Organization chart NRC
Head
Mieke Reyniers
Plant DiseasesMaikel Aveskamp
- Bacteriology- Mycology- Virology
Plant PestsAnton van der Sommen
- Entomology- Nematology- Invasive plants- Centre for Monitoring vectors
Diagnostic methodsErik Souer
- Administration- Biosafety/-security- Molecular Biology- Greenhouses
National Reference Center – plant healthmore than a lab
Tasks of the NRC
Knowledge
NRC has an independent, scientific based position within the NVWA
Contribution to science: diagnostic
methods, diagnostic protocols, taxonomy, biology and epidemiology
Supervision/contribution to research projects: eg H2020 (EU), EUPHRESCO (EPPO), PPS (Min. EZK)
Knowledge
Working on pest risk analysis, quickscans (EU, EPPO, EFSA)
Participation in International meetings
Maintenance of network (lab visits)
Methods
Method development:
1) Test Development literature, third parties, own research
2) (Lab-)ValidationRobust, specific, reliable
3) Implementation
Diagnosis
- Clients:- NVWA inspection- Agricultural Inspection Agencies
(reference samples)- Third parties
Mainly focus on quarantine-organisms
(risk-based)
Diagnosis
- Microscopy(Conventional, IF, EM)
- Biochemical tests (eg DAS-ELISA, MALDI-TOF)
- In planta(e.g. Koch’s Postulates, fysiodetermination)
- Molecular methods(eg RealTime PCR, Sangersequencing
new: High Throughput Sequencing)
Identification = Combination of at least two independent tests
Facilities
Quarantine building
Quarantine greenhouses
Outdoor fields (open and contained)
Diagnosis
Collections
- Collection ~ 230k specimens of plant pests and pathogens(Reference material)- Herbarium/ pinned insects /
microscopic slides- Living collection- On culture- Symptoms- Tissue and DNA
Education
- Training (inspectors of NVWA, inspectors
of Agricultural Inspection Agencies, EU, public networks, EUPHRESCO)
- Capacity building and Twinning (eg Turkey, Vietnam, Egypt, Kenya)
- Internships(applied-) universities, college
Advice
NVWA, Ministryeg Risk assessments, Legislation 3rd countries (export), elimination scenarios, …
InspectionSampling methods, risk-based inspection,…
EPPO, EU, IPPCDiagnostic protocols, Methodologies, …
Supervision
Labs of the Agricultural Inspection Agencies (NAK, Naktuinbouw, BKD, KCB)
- Training- Advice- Auditor: ISO 17025 Quality Management implementation
All labs working with Q-organisms- Auditor: 2008/61 Biosecurity implementation
Potential threats:
Signaling (literature/finding/third country)
Quick-scan
Pest Risk Analyses (PRA)
Risk Assessment
Quality Management System
- ISO17025 accredited
- Fixed scope (since 2014)Accreditation is limited to predefined host-matrix combinations
- Flexscope (since 2017)Extension of accreditation is possible for allhost-matrix combinations
www.rva.nl
Biosafety & Biosecurity
- InternalMake sure that organisms stay inside
- ExternalAdvise on working with Q-organisms under 2008/61 - labs of the Agricultural Inspection Agencies- other phytosanitairy labs,- breeding companies, - research institutes, - universities
Goals workshop:- expanding professional network- sharing knowledge via presentations and posters- identify shared research needs