VII edizione Capri PediatriaCapri,28 – 30 Aprile 2014

Biomarkers nell’asma bronchiale

Guglielmo ScalaNapoli

Pathogenesis of AsthmaHolgate S, Clin Exp Allergy, 2008

Pathogenesis of AsthmaHolgate S, Clin Exp Allergy, 2008

La cellula Th2 è fondamentale per la maturazione dell’eosinofilo

Pathogenesis of AsthmaHolgate S, Clin Exp Allergy, 2008

A new look at the pathogenesis of asthmaHolgate ST, 2010, Clinical Science

A new look at the pathogenesis of asthmaHolgate ST, 2010, Clinical Science

Pathogenesis of AsthmaHolgate S, Clin Exp Allergy, 2008 Nasce il

concetto di EMTU

Lamina basale è prodotta dalle cell epiteliali

Lamina reticolare (MBR) è prodotta dai fibroblasti

A new look at the pathogenesis of asthmaHolgate ST, 2010, Clinical Science

Viral infections and atopy in asthma pathogenesis: new rationales for asthma prevention and treatmentP. Holt & P. Sly, Nature Medicine, 2012

Holt & Sly, Nat Med 2012

Holt & Sly, Nat Med 2012

Holt & Sly, Nat Med 2012

Holt & Sly, Nat Med 2012

Holt & Sly, Nat Med 2012Smascherata la marcia atopica??

The basics of epithelial-mesenchymal transitionKalluri R, Weinbergjj R , J Clin Invest. 2009.

The basics of epithelial-mesenchymal transitionKalluri R, Weinbergjj R , J Clin Invest. 2009.

A clinical pharmacological study of the potential beneficial effects of a propolis food product as an adjuvant in asthmatic patients.

Khayyal MT 2003, Fundam Clin Pharmacol

FVC + 19%

FEV1 +30%

PEFR +30%

FEF 25-75 +41%

TNF -52%

ICAM-1 -65%

IL-6 -44%

IL-8 -30%

IL-10 +300%

Asma notturno/settimana - 60 %

22 pazienti : placebo24 pazienti : propoli

Brazilian Green Propolis: Anti-Inflammatory Property by an Immunomodulatory Activity Machado JL et al, 2012, Evidence-Based Compl Altern Med

Caffeic acid phenethyl ester inhibits nuclear factor-kB and protein kinase B signalling pathways and induces caspase-3 expression in primary human CD4+ T cells

Wang et al, 2009, Clin Exp Immunol

The inhibitory effect of CAPE results in the inhibition of cytokine production and proliferation in primary human CD4+ T cells

Caffeic Acid Phenethyl Ester and Its Amide Analogue Are Potent Inhibitors of Leukotriene Biosynthesis in Human Polymorphonuclear Leukocytes. Boudreau L, 2012, PlosOne

Caffeic Acid Phenethyl Ester and Its Amide Analogue Are Potent Inhibitors of Leukotriene Biosynthesis in Human Polymorphonuclear Leukocytes. Boudreau L, 2012, PlosOne

Propolis inhibits TGF-β1-induced epithelial-mesenchymal transition in human alveolar epithelial cells via PPARγ activation. Kao HF, et al. 2013, Int Immunopharmacol

TGF- bAlterazioni morfologiche↓ E - cadherin↑ N - cadherin↑ ROS↑ motilità cellulare

Propolis inhibits TGF-β1-induced epithelial-mesenchymal transition in human alveolar epithelial cells via PPARγ activation.

Kao HF, et al. 2013, Int Immunopharmacol

TGF- bAlterazioni morfologiche↓ E - cadherin↑ N - cadherin↑ ROS↑ motilità cellularePROPOLI

Se pretrattiamo con CAPE (ac. Caffeico) l’effetto è di minore intensità.

Propolis inhibits TGF-β1-induced epithelial-mesenchymal transition in human alveolar epithelial cells via PPARγ activation. Kao HF, et al. 2013, Int Immunopharmacol

The results of this study suggest that natural propolis extracts may prevent TGF-β1-induced EMT in alveolar cells, which may be clinically applied in the prevention and/or treatment of EMT-related fibrotic diseases as well as airway remodeling in chronic asthma

guglielmoscala@gmail.com