Urinary Tract System - JU Medicine · lactose fermenter o Swarming motility ( flagellated ) o...

Transcript of Urinary Tract System - JU Medicine · lactose fermenter o Swarming motility ( flagellated ) o...

Urinary Tract System

• Early morning midstream urine sample.

• Cultured on Macconkey and blood agar .

• Small loop 1 µl count colonies* 1000(0.01 ml)• Large loop 10µl count colonies* 100(0.1ml)• 10 ^5 > significant infection.• 10^3-10^5 intermediate infection.• 10^3 < Not significant.• Colony count unite : CFU (colony forming unite).

(E.coli) 1. Escherichia coli

2. Klebsiella

o Gram negative rods , nonlactose fermenter

o Swarming motility ( flagellated )

o Prevent swarming by culturingit on CLED or Macconkey media

Proteus

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjprsqb4rnXAhVQJOwKHa6DCYcQjRwIBw&url=http://atlas.sund.ku.dk/microatlas/veterinary/bacteria/Proteus_mirabilis/&psig=AOvVaw0FB1C5npq9JPBklNWkfpRw&ust=1510601155849865

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwiNhd7e5LnXAhVJoKQKHZctBS0QjRwIBw&url=https://microbewiki.kenyon.edu/index.php/Swarming_Motility_in_Proteus_Mirabilis:_Causative_Agent_of_UTIs&psig=AOvVaw0FB1C5npq9JPBklNWkfpRw&ust=1510601155849865

Biochemical tests for E.coli

1) kligar sugar iron:

• For E.coli :

Acid/acid (A/A)(Y/Y)

Gas +

No H2S

Kligler test

2) Citrate test :

Negative for E.coli

3) Urease test :

Negative for E.coli

4) SIM (SULFIDE, INDOLE, MOTILITY) MEDIUM

Indole positiveSulfide (H2S) = negativeMotility =motile

Neg Pos

identified by :

•Gram negative rods

•odor : grape-like smell.

•Oxidase positive.

•Produce different pigments colors:• Pyocyanine (blue-green)

• Pyoverdin (fluorescent yellow –greenish pigment).

• Pyorubrin (red).

• Pyomelanin (brown).

Pseudomonas aeruginosa

Pseudomonas aeruginosa on mueller hintion agar

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjo8PLCvubWAhVFI8AKHYsLAm4QjRwIBw&url=http://ansalshah.blogspot.com/2013/01/dissecting-worst-plot-of-strong.html&psig=AOvVaw042x8IW2OgXu-mGV9vCE2e&ust=1507739698806809

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjy2a32vubWAhVMB8AKHWWBAO0QjRwIBw&url=https://nl.pinterest.com/pin/345440233908669327/&psig=AOvVaw2ZVhlYpekhkNGLpq9wPTOH&ust=1507739888370647

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwi0u9Ktv-bWAhXnIMAKHU8pA-4QjRwIBw&url=http://arushad98.yoo7.com/t4003-topic&psig=AOvVaw25CG2kR4gCh9fCA8BeUub3&ust=1507739957243253

Blood agar

A- staphylococcus .albus

B- staphylococcus.Aureus

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwiCl-CUwubWAhVoIsAKHQxUAxMQjRwIBw&url=http://www.advbiores.net/article.asp?issn=2277-9175;year=2014;volume=3;issue=1;spage=32;epage=32;aulast=Rit&psig=AOvVaw0gnhNHyQRXFVCjzE9oBY00&ust=1507740739185792

CLED media Lactose fermentation ( +ve )

staphylococcus spp.

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjSwJnZxObWAhWCA8AKHYAECo0QjRwIBw&url=http://www.imgrum.org/user/medical_microbiology/1986611985/1031655022512769911_1986611985&psig=AOvVaw13ll8iUw2fX4w0DQ5SLdXd&ust=1507741418271961

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjv8cy41ebWAhXIDMAKHeGXDmcQjRwIBw&url=http://www.medical-labs.net/hektoen-enteric-agar-2204/&psig=AOvVaw0eMvb29g22SlkF5-To5pKv&ust=1507745926696468

Test for differentiation of Staphylococcus species

Catalase test

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwid0YDE5-bWAhVnIMAKHZCoB64QjRwIBw&url=https://www.studyblue.com/notes/note/n/lab-exam/deck/12733084&psig=AOvVaw343DWlqYvsdCDF0mi3dqrv&ust=1507750751980608

MSAMannitol salt agar media

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwj32KyZ6ebWAhXrK8AKHY9bB8MQjRwIBw&url=https://www.xebios.com/items/mannit-kochsalz-agar-nach-harm-ep-usp-jp&psig=AOvVaw01yq077G-LcozYq2SAB0er&ust=1507751220256405

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjb0PrQ6ebWAhVFGsAKHWzUBhIQjRwIBw&url=https://www.researchgate.net/figure/235730553_fig3_Mannitol-salt-agar-plate-with-oxacillin-showing-methicillin-resistant-Staphylococcus&psig=AOvVaw01yq077G-LcozYq2SAB0er&ust=1507751220256405

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwiZnrnp6ebWAhXlJsAKHXwrDJMQjRwIBw&url=http://faculty.ccbcmd.edu/courses/bio141/labmanua/lab15/msase.html&psig=AOvVaw01yq077G-LcozYq2SAB0er&ust=1507751220256405

S.aureus

S.albus

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjx5ojj6-bWAhWFIsAKHcnoATUQjRwIBw&url=http://intranet.tdmu.edu.ua/data/kafedra/internal/micbio/classes_stud/en.../med/lik/ptn/Microbiology, virology and immunology/2/05_Genetics of bacteria..htm&psig=AOvVaw1qurHC2M2t7ztTPYfjRK9k&ust=1507751808693816

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=&url=http://hematology-lectures.blogspot.com/2015/11/prothrombin-time-pt-manual-test.html&psig=AOvVaw2tENBnB1vYvAmm2cn7A9Zi&ust=1507751975900354

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwje75mr7ubWAhVnDMAKHYv2CdsQjRwIBw&url=http://colors.ga/nepew/beta-strep-group-b-bactrim-1467.php&psig=AOvVaw3JJTkTyC5M-jIuVdUJ82LW&ust=1507752535275339

Bile Esculin Test

Neiserria

Intra cellular

Extra cellular

Gram

Negative

diplococci

Neisseria

IntracellularExtracellular

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0CAcQjRxqFQoTCMeCkraR78gCFYe4FAodpQEKfg&url=http://www.msevans.com/cnsinfections/neisseria.html&psig=AFQjCNGhHOaNizWKJuDaaMyvGpMZw8hdYg&ust=1446464229801896

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0CAcQjRxqFQoTCJPz1MuS78gCFQq2FAodkNoCeA&url=http://imgarcade.com/1/neisseria/&psig=AFQjCNFXM4qauI-QadTTDtEbd64j0PsNWg&ust=1446464499989492

Haemophilius ducreyi (cancroid)

Schistosoma haematobium worms

Schistosoma haematobium egg

Trichomonas vaginalis parasite

Treponema pallidum

Candida Species

Candida

Spp.

C.albicans

C.tropicalis

C.glabrata

C.Krusei

Candida Spp

• Larger than Bacteria

• Budding

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwi08c7Tv8vXAhVGL1AKHamACl8QjRwIBw&url=https://microbeonline.com/candida-albicans-pathogenesis-diagnosis/&psig=AOvVaw0mBnoaR5rDR-IVK0Qa3GIl&ust=1511210414146854

sabouraud dextrose agar

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwixg773wcvXAhVDZFAKHVzcDcgQjRwIBw&url=http://www.pouringindia.com/sabouraud-dextrose-agar-plates.htm&psig=AOvVaw0cO_9tr6m4mRheyKTRoJ7o&ust=1511211029232224

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjxnqiDwsvXAhXRaFAKHbPdBdQQjRwIBw&url=http://yeastinfectioncause.net/2013/09/29/candida-albicans-on-sabouraud-dextrose-agar-3/&psig=AOvVaw0cO_9tr6m4mRheyKTRoJ7o&ust=1511211029232224

To Differentiate between C.albican and other Species

Germ tube[ Serum + candida ]

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjb25i-wMvXAhXFfFAKHcORA5QQjRwIBw&url=https://microbiologyinfo.com/germ-tube-test-principle-procedure-results-interpretation-and-limitations/&psig=AOvVaw2mwqAntE0Mkr8GULFd9K6d&ust=1511210603812760

2. Chrom agar

C.glabrata : violet (dark pink)

glistering C.albicans : Green

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwjMx6n9w8vXAhXDI1AKHZycAzoQjRwIBw&url=https://twitter.com/chromagar&psig=AOvVaw3YxoL6ZYBhXvh7OD6L0vrb&ust=1511211527108856

C.TropicalisBlue

C.Krusei : rough dry pale pink

Chrom agar

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwj-v_X2wsvXAhVQY1AKHdoBCCkQjRwIBw&url=http://www.chromagar.com/products-chromagar-stec-focus-on-stec-e-coli-51.html&psig=AOvVaw1b4m8ZBsgSZi0KgKpYYzkM&ust=1511211233334434

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwith5zUw8vXAhWIL1AKHfEKCywQjRwIBw&url=http://www.jdrntruhs.org/article.asp?issn=2277-8632;year=2012;volume=1;issue=4;spage=222;epage=226;aulast=Sukumaran&psig=AOvVaw1bmSoVou4fan1O0H1XqPqP&ust=1511211476052066

http://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwi7m7PLx8vXAhUSalAKHUstCsUQjRwIBw&url=http://www.e-jams.org/article.asp?issn=2249-4855;year=2012;volume=2;issue=3;spage=101;epage=104;aulast=Shettar&psig=AOvVaw3ifa5jaFY6ZewgCNOXCcIm&ust=1511212545142790

https://www.google.jo/url?sa=i&rct=j&q=&esrc=s&source=images&cd=&cad=rja&uact=8&ved=0ahUKEwiAp52A7rnXAhVN2aQKHXBZAdYQjRwIBw&url=https://www.slideshare.net/ProphetsAgency/trends-in-interactive-design-2013/147-Thank_you_for_listeningQUESTIONS_ALWAYS&psig=AOvVaw1irK4epExuPAK--fH3GpXc&ust=1510604323778298

Urinary Tract System - JU Medicine · lactose fermenter o Swarming motility ( flagellated ) o...

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