Triplet Blinking stable triplet state S 1 T 1 due to solvent halogens transition metals

QuickTime™ and aTIFF (Uncompressed) decompressor

are needed to see this picture.

Triplet Blinking- stable triplet state- S1 T1 due to solvent

-halogens-transition metals

Transition to the Dark Side

Collision with solutes

Resonance energy transfer

Quenching

Energy transferredto non-fluorescentsolute molecule

Fluorescence Resonance Energy Transfer

r < 50 A

Photobleaching

T1 is highly reactive

O2 likes T1 electrons

Oxygen radicals are highly reactive & cytotoxic

Death of a Fluor

O2 or other solute New compound(disrupted conjugation)

Reflected Light (Epifluorescence) Microscopy

Transmission Fluorescence Microscopy

Widefield Fluorescence Microscopy

Confocal Fluorescence Microscopy

Marvin Minsky’s Confocal Microscope

Patent application submitted in 1957 while Minsky was a post-doc at Harvard

Epi-illuminated design eliminated need for a condenser

Also incorporated the concept of stage scanning

ConfocalMicroscope

Invented in 50s

Not practical until availabilityof low cost lasers and high speed computers

First commercial devices available in the 80s

Uses pinhole to limit collected light to that which is “confocal” (i.e., shares conjugates planes of focus) with the object plane

Zeiss LSM510

Reduced blurring of image by light scattering

Increased effective resolution

Improved signal-to-noise ratio

XY-scan possible across a wide area of the specimen

Inclusion of Z-scan possibility

Electronic adjustment of magnification

Possibility of quantitative measurements of optical properties of the specimen

Nipkow Disk

Invented in 1884 by Paul Nipkow,a contemporary of Abbe.

Central element of an early incarnation of the television.

Tandem Scanning Optical Scopes

Light Amplification by Stimulated Emission of Radiation

1917 Albert Einstein stimulated emission possible

1950s Charles Townes built first “MASER”

Gordon Gould coined term LASER

1960 Theodore Maiman built first LASER (ruby)

Stable LASER outputrequires establishment and maintenance of a “population inversion” of excitable electrons.

Must “pump” the medium with either electrical or photonic energy

~ 10,000 - 40,000 hours

Laser Lines

Alignment

Triplet Blinking stable triplet state S 1 T 1 due to solvent halogens transition metals

Documents

Transcript of Triplet Blinking stable triplet state S 1 T 1 due to solvent halogens transition metals

Group VII elements - Halogens

Group 7 Halogens

Singlet-Singlet and Triplet-Triplet Energy Transfer in ... · PDF fileSinglet-Singlet and Triplet-Triplet Energy Transfer in Bichromophoric Cyclic ... constructed by a solid phase

Our family: The Halogens

Group 17 -The Halogens

Frequent Blinking

Halogens part 2 chemical properties

Halogens in the Troposphere - SOLAS INT · HitT – Halogens in the Troposphere - is to determine the importance of RHCs ... Halogens in the troposphere 5 2) Halogen domains Historically,

s&p elements Halogens

Halogens - University of Michigan

Correcting the Triplet Selection Bias for Triplet Lossopenaccess.thecvf.com/content_ECCV_2018/papers/...Correcting the Triplet Selection Bias for Triplet Loss Baosheng Yu1, Tongliang

17 - Halogens

Halogens (group 17 )

New perspectives for triplet–triplet annihilation based ...

Kinetic Monte Carlo study of triplet-triplet annihilation ...

KS4 Halogens

The Halogens ANORGANIK

Direct observation of triplet energy transfer from ... · triplet-triplet energy transfer (TTET) by moni-toring the kinetic growth of the characteristic triplet-to-triplet (T 1 →

on blinking

Blinking Lights h407