The new ICBP animal core facility

Ioana Madalina Fenyo, PhD

SPF Animal Core Facility Coordinator

April 23, 2015

Conventional area

SPF Area(Experimental)

SPF Area(Breeding)

Washing area

Quarantine

Platforma de Modelare Experimentala

SPF area - 1215 mouse cages and 258 rat cagesBreedingExperimentalBSL-2 lab (transduction)

Conventional area - 144 hamster/rat cagesBreedingExperimental

SPF Area(Experimental)

SPF Area(Breeding)

IVC racks (Allentown) laminar-flow exchange stations (Allentown)

SPF Area(Experimental)

2 surgery rooms:

- aspirating surgery tables

- Stemi 2000-C stereomicroscopes (Zeiss) with

Digital Microscopy Cameras AxioCam ERc 5s

- gas anesthesia induction systems (Minerve)

temperature regulation module (Minerve)

- CO2 chambers (Minerve)

- light sources (Harvard Apparatus)

- tail vein injection unit (Kent Scientific)

- PowerLab physiological monitoring equipment

(ADInstruments)

- small sterilizer for instruments (Matachana)

- ventilators (Harvard Apparatus, Ugo Basile)

- homeothermic blankets (Panlab)

- microfuge (Hettich)

SPF Area(Experimental)

Vevo 2100® High-resolution Imaging System (VisualSonics)

SPF Area(Experimental)

IVIS Spectrum in vivo Imaging System (Caliper, PerkinElmer)

SPF Area(Experimental)

irradiation unit (RS2000 Rad Source X-ray irradiator)

Leica AM 6000 platform for micromanipulation applications (Leica/Eppendorf)

NSG

Inbred

C57Bl/6

FVB

CBA

Geneticaly modified

apoE KOGSH

Outbred

Wistar

linia TRE-hapoE3

linia Tek-Tet3G

UN MODEL TRANSGENIC, DE TIP CONDIŢIONAL, PENTRU INDUCEREA CONTROLATĂ

A EXPRESIEI APOE3 UMANE ÎN MOD SPECIFIC ÎN ENDOTELIUL VASCULAR

Crearea unui model care să ofere posibilitatea investigării funcţiei apoE în

ateroscleroză şi a modului în care o creştere a concentraţiei acestei proteine la nivelul

plăcii, dar şi în circulaţie, poate ameliora procesul patologic.

Principiul transactivării transcripţionale dependente de tetraciclină – sistemul “tet-on”

Tek/Tie2promoter-Tet3G-Tek/Tie2enhancer(Tek-Tet3G)

TRE-hapoE3

linia TRE-hapoE3 linia Tek-Tet3G

AcknowledgementsOur staff

Conventional area

Emanuel Dragan, VMD – coord.

Emilian Dunana

Lacramioara Costea

SPF area

Madalina Fenyo, PhD – coord.

Safta Nae

Genia Ruse

Paunica Cojan

Serban Morosan, DVM, PhD – UPMC

Francois Lachapelle, PhD

Sebastien Dussaud, PhD

Christelle Enond

Yoann Pain

Dr. Anca Gafencu

CARDIOPRO (143 / SMIS CSNR 2667)

THANK YOU!

AC 1400 washer disinfector (Steelco)

baby bottle washer disinfector with filling station (Steelco)

DS-500 Dump Station (Allentown)

Washing area

Washing area

Two-door autoclave (Matachana) Chemical SAS (Matachana)

Quarantine

InnoSte transfer isolator (EuroBioConcept)Animal transfer station (Allentown)

InnoSte isolator (EuroBioConcept)Animal transfer station (Allentown)