Post on 03-Jan-2016
• SLO: Apply previously learned technique to life cultures
Ex. 3: Staining Techniques - Gram Stain, part 2 (Live Cultures)
Smear Preparation
From Liquid Medium
• Suspend bacterial sediment in culture tube by gently tapping on it.
• Transfer one loopful of the bacterial suspension to slide and spread out (You may exceed the limits of the circle)
• Air-dry and heat-fix
From Solid Medium
• Transfer one loopful of H2O into circle area of slide.
• Using loop, suspend very small quantity of colony of interest in that drop of water and spread the suspension evenly. It should only appear slightly milky. Do NOT use too many bacteria
• Air-dry and heat-fix
Artifacts Precipitated crystal violet stain may resemble cocci. Clues: dense clumping, small size of the gram-positive dots. Paucity of organisms elsewhere in the field.