In vitro Assessment of Antimicrobial Activity of some

Ornamental Flowers

Presented by:Bidisha Ghosh8th Sem, B.Tech, Biotechnology, SITM

Under the supervision of:Mr. Neeraj Mishra

Asst. Prof. & Head(Dept. of Biotechnology), SITM

Infectious disease & traditional drug problems

Leading cause of premature deaths, killing almost 50,000 people/day

Just 4yrs after the start of mass-producing penicillin in 1943 resistant microbes began appearing

Development of resistance to common antibiotics in all major pathogens are reported

These negative health trends necessitated a search for new antimicrobial substances from other sources including plants.

Use of flowers as medicine

50% of pharmaceuticals originate from plants

Most plants contain bioactive compounds that have good antimicrobial activity

Plant antimicrobials are not associated with side effects

Some flowers with reported antimicrobial

activity:

ChrysanthemumJasminePoppyRosemaryThyme

Objective of project

Evaluating antimicrobial activity of water & alcohol extracts of 4 flowers against 5 microorganism by Agar Well Diffusion Method.

Flowers used

Cyme rose (Rosa indica)

Lotus(Nelumbo nucifera)

Orange Marigold(Tagetes erecta)

Yellow Red Marigold(Tagetes patula)

Preparation of flower extracts

Water

extract

5 g flower+

100 ml dist. water

Ran in soxhlet

(4 hrs at 100OC)

Alcoholextract

5 g flower+

100 ml alcohol

agitated for 8 hrs in a wrist-action

shaker

alcohol evaporated without heat & residue

mixed with 100 ml of dist water

Microorganisms analyzed

Bacillus subtilis

Staphylococcus aureus

Escherichia coli

Pseudomonas aeruginosa

Candida albicans

Analysis of antimicrobial activity

Method:

Agar Well Diffusion Method

Media:

Muller Hinton agar

Parameter:

Zone of Inhibition Measuring the inhibition zone

Antimicrobial activity against Staphylococcus aureus

Water extract

Alcohol extract

0

2

4

6

8

10

12

Bacillus subtilis Staphylococcusaureus

Escherichia coli Pseudomonasaeruginosa

Candida albicans

inhib

ition z

one(in m

m)

Rosa indica Nelumbo nucifera Tagetes erecta Tagetes patula Control

Graphical representation of antimicrobial activity with water extract

Orange Marigold showed activity against all five microorganisms

Rose gave the highest inhibition.

Candida albicans showed the highest resistance & was susceptible only to Orange Marigold & Yellow Red Marigold.

0

2

4

6

8

10

12

Bacillus subtilis Staphylococcusaureus

Escherichia coli Pseudomonasaeruginosa

Candida albicans

inhib

ition z

one (

in m

m)

Rosa indica Nelumbo nucifera Tagetes erecta Tagetes patula Control

Graphical representation of antimicrobial activity with alcohol extract

Yellow Red Marigold showed very high activity against all five

organism

Rose also showed strong inhibiting capability

Most resistant microorganism was again Candida albicans

ConclusionConclusion

Flower extracts have great potential as

antimicrobial compounds

Rosa indica & Tagetes patula showed

maximum antimicrobial activity

These flowers can be used to discover bioactive compounds that may serve as leads for development of new pharmaceuticals

AcknowledgementsAcknowledgements

Mr. Neeraj Mishra, Asst. Prof. & Head of the

Dept., Biotechnology, SITM.

Dr. Vinod Bihari, Director of Department of

Biotechnology All the faculty of the Dept., Biotechnology,

SITM. My parents.

ThankThank

YouYou