Post on 17-Jan-2016
description
Project Idea: Biofuels
Stanford iGEM 2009
Presentation by:Suzie
+Robert
3/4/09
Biofuels Introduction
• What makes a good biofuel?!?!
• Environmentally friendly
• Mass production - CHEAP!
• Chemically:
• High energy density
• Low water solubility
Biofuels Introduction
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Biofuels Introduction
• As for synthetic biology applications…
• Most use E. coli or S. cerevisiae as a host to:
1. Engineer amino acid biosynthesis
2. Engineer fatty acid pathways
3. Engineer isoprenoid pathways
Dr. James Liao
QuickTime™ and a decompressor
are needed to see this picture.
Dr. James Liao
QuickTime™ and a decompressor
are needed to see this picture.
3-Methyl-1-Butanol
QuickTime™ and a decompressor
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2-Methyl-1-Butanol
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Dr. James Liao
Our Project
• We would need to engineer a separate amino acid pathway to produce a possible biofuel
• EX: Serine or alanine• Need to ID enzymes that can make this
possible:• For Dr. Liao, it was KIVD (yeast) and
ADH6 (lactococcus lactis)
Five Possible Sub-projects
1. Synthesizers (constructing the pathway)
2. Riboregulators (controlling knockouts)
3. Modelers (Modeling for bottlenecks)
4. Bottleneckers (Building expression system)
5. ANY OF THE ABOVE