Post on 21-Dec-2015
Prion Project - UpdateMass Spectrometry of Prions
Andy Lo, Béla Reiz, Messele Fentabil
Dr. Liang Li
Department of Chemistry
University of Alberta
Mass Spectrometry (MS)
Mass Spectrometry is an instrumental method used to identify chemical substances by means of the separation of their gaseous ions using magnetic and/or electric fields according to their mass-to-charge ratio.
MS in PROTEOMICS
Molecular weight of proteins
Masses of peptides in a mixture
Masses of fragment ions of a peptide
Experimental Procedure
Protein
Digestion (Peptides)
LC-MALDIMS
MS
MSMS
MS
Database Search
Protein MS
Experimental Procedure
Protein
Digestion (Peptides)
LC-MALDIMS
MS
MSMS
MS
Database Search
PrP Tryptic Digest - MS
PrP&Myo Tryptic Digest - MS
Experimental Procedure
Protein
Digestion (Peptides)
LC-MALDIMS
MS
MSMS
MS
Database Search
PrP&Myo Tryptic DigestLC-MALDI-MS
Experimental Procedure
Protein
Digestion (Peptides)
LC-MALDIMS
MS
MSMS
MS
Database Search
PrP&Myo Tryptic DigestLC-MALDI-MSMS
Experimental Procedure
Protein
Digestion (Peptides)
LC-MALDIMS
MS
MSMS
MS
Database Search
Database Search Results
Database Search Results
Database Search Results
Database Search Results
PrP Chymotryptic DigestLC-MALDI
PrP Chymotryptic DigestResults
PrP Chymotryptic DigestResults
PrP Chymotryptic DigestResults
PrP Chymotryptic DigestResults
Future Work
Chemical surface modification of surface exposed residues using:
N-bromosuccinimide (NBS) for Trp exposed residues
Hydroxylation of surface exposed Cys, Trp, Tyr, Met
Thanks!
Dr. Liang Li Andy and Messele All Li Group members