DEPARTMENT OF ZOOLOGYWELCOMES

M.V.P.Samaj’s K.T.H.M. COLLEGE,

GANGAPUR ROAD, NASHIK – 422 002

TOXICITY EVALUATION OF PYRIDONE DERIVATIVES ON Sitophilus Oryzae Linn

ByMrs. Jayshree Vitthal Shinde

Under the guidance of

Dr. A. E. Desai

INTRODUCTION

• Insect existed at least 20 million yrs ago.• Grain pests e.g. Rodents, Nematodes, Fungi• Classification of pests• Plants extracts/ botanical insecticides • Synthetic chemical compounds

Aim Of This Project

• Toxicity Evaluation of Synthesized Chemical Compounds on Stored Grain Pests.• To Protect Stored Grains.

Material & Methods

• Collection & Culture Of Sitophilus oryzae Linn

• Synthesis of chemical compounds.

Observation Table

Table no 1:

2,5dihydro-7,8dimethoxy-4(3-methoxyphenyl)-2-oxo-1H-in deno (1,2-b)pyridine-3carbonitrile.

Sr.no.

Dose in ppm

No.of insects released

Mortality for

6hrs 12hrs 18hrs 24hrs 1 5 10 1 2 3 6 2 10 10 2 3 3 7 3 15 10 2 3 4 8 4 20 10 3 3 4 9

Table no 2:

2,5dihydro-7,8dimethoxy-4(3,4-dimethoxyphenyl)-2-oxo-1H-in deno (1,2-b)pyridine-3carbonitrile.

Sr.no.

Dose in ppm

No.of insects released

Mortality for

6hrs 12hrs 18hrs 24hrs 1 5 10 2 2 2 5 2 10 10 2 3 3 6 3 15 10 3 3 3 8 4 20 10 2 3 4 9

Table no 3:

2,5dihydro-7,8dimethoxy-4(3,4,5-trimethoxyphenyl)-2-oxo-1H-in deno (1,2-b)pyridine-3carbonitrile.

Sr.no.

Dose in ppm

No.of insects released

Mortality for

6hrs 12hrs 18hrs 24hrs 1 5 10 1 2 3 6 2 10 10 2 3 3 8 3 15 10 2 3 4 9 4 20 10 3 3 3 9

Table no 4:

2,5dihydro-7,8dimethoxy-4(thiophen-2-yl)oxo-1H-in deno (1,2-b)pyridine-3carbonitrile.

Sr.no.

Dose in ppm

No.of insects released

Mortality for

6hrs 12hrs 18hrs 24hrs 1 5 10 2 2 3 6 2 10 10 2 3 3 7 3 15 10 2 3 4 9 4 20 10 3 2 3 9

Result

Chemical Time in LC50 ppmRegression eqnHeterogencityVarianceFinancialComp Hrs. ± S.E. Y-a+b x2 (n-1) Limit

0 5 10 15 20 A=y-bx

Comp I 24 0 60 70 80 90 1.5515 ± 0.99824.438 2.46 0.997 m1=0.0951(101) m2=3.0075

Comp 2 24 0 50 60 80 90 1.3357 ± 0.73543.908 -2.539 0.54 m1=0.1044(104) m2=2.567

Comp 3 24 0 60 80 90 90 1.5885 ± 0.95674.447 2.983 0.905 m1=0.1034(105) m2=3.0736

Comp 4 24 0 60 70 90 90 1.5134 ± 0.86544.315 2.936 0.802 m1=0.0311(106) m2=2.9957

Table No. 9 - Comparative Results

Mortality

Graph

Discussion

• There is significant increase in mortality from low to high dose of chemical compound.

• S. oryzae, R. dominica, T. castaneum shows high mortality when exposed to biogass in PVC bins (Bennett, 2003).

• Azadirachtin exhibited good efficiency against Musca nobulo L, Culex fatigans, Tribolium castaneum (Paul et, al.1963).

• PN101, LC50=1.5515+0.9982, PN104, LC50=1.3357+0.7354, PN105,LC50=1.5134+0.8954, PN106, LC50=1.5885+0.9567.

References

• Bennett S. (2003). Stored Product insects, available on: www.thepiedpiper.co.uk/th7.htm. (Accessed 22march 2012)

• Finney D.(1971). Probit analysis Cambridge University press, London.

• Paul, C.F, R.S.Dixit and P.N. Agarwal. (1963). Evaluation of the insecticidal properties of the seed oil and leaf extract of the common Indian neem, Azadirachta indica L. Sci. Cult. 29:412-413

Thank You