Post on 06-Feb-2016
description
Preparing client to microbe inspection
Oleh :
Dr. Jacky Junaedi
Introductiom
Kind of specimen sputum, blood, feces, and urin
At least one time did
What for?- diagnosis- bacteria/virus/parasite, etc- terapeutic responses
SPUTUM
SPECIMEN REMOVAL SPUTUM
The best in the morning Brushed teeth first Rinse the mouth Cough to get the sputum 15-30cc, First cough sputum
PlacedSaving less than 24 hour in room
temperatureSterrile pot placed
ShippingShipping < 2 hour in room
temperatureTransport medium (Amies
medium, Stuart’s medium)
STUART’S MEDIUM AMIES MEDIUM
Blood specimen inspection
Blood specimen:
Hematology inspection Chemical inspection
Serologin and immunologic inspection
Parasitologic and blood cultureWIDALTEST
Swab the vein superficial place with 70% alcohol
apply the torniquet 45 degree manuver of spuit, pull the
blood Put the blood to the liquid media TSB
(Trypticase Soy Broth)
SPUIT
TSB
Specimen removal
Vein blood
10-20cc
The best at febris
PlacedSaving less than 24 hour in room
temperatureSterrile placed
ShippingShipping < 2 hour in room
temperature
feaces specimen inspection
Kind of inspection Bloody feaces????? Parasite egg Bacterial andn virus
Specimen removal
handscoon Amount of feaces
2,5 cm solid feaces 15-30 mL liquid feaces
For feaces culture use a sterrile swab
SWAB
Placed Less then 1 hour in room temperature
Stuart’s medium, ataupun Pepton water Saving < 24 jam room temperature, > 24 jam at
4°C
Shipping Less then 1 hour in room temperature
STUART’S MEDIUMPEPTON WATER
TETRA THIONATE BROTH
Urine specimen inspection
(clean-catch or midstream urin specimen)
Urinalisis and microbe inspection
KATETER
Specimen removal:
Clean up the oue (penis or vagina)
Collect the mid stream urine
30-60mL
Place and shipping
Less then 1 hour at room temperature
Refrigerator store less then 24 hours
Use urine transport tube (sterrile)
STERIL CONTAINER
URINE TRANSPORT TUBE