DNA damage

Growthfactor

Siah1

HipK2

Tip60

Set7/9

Chk1

DAPK

CK1

DNA-PK

ATR

p38ERK

MK5/PRAK

AMPK

p38

TAG

PP1

Wip1

ATM

acK

acK

CARM1

PRMT1

p18/hamlet

cdk8

Axin

BRN-3b

LSD1

Pin1

iASPP

YY1

ASPP1/2

p52

p66shc Pig3 Pig6

Bax Bak

Bcl-2 IAP1 IAP2

Blk Puma Noxa

CDIP DR5 FAS

maspin TSP1 BAI-1

GADD45A 14.3.3s FBXW7

XPC p53R2 DDB2

PHLDA3 PTEN IGFBP3

Dram

gpx1 Aldh4 p53Dinp1

BTG2 p21 PAI-1 Dec1

AMPKb1/2 Sestrin 1/2 Redd1 TSC2

Tigar GLS2 GAMT Sco2

HDAC1

Sin3AATM

Sirt1

pCAF

PML

p68

BRCA1

BRN-3achk2

PRMT5

JMY

E4F1

Hzf

hnRNPK

p300/CBPMdmx

Smyd2

Set8

chip

ARFBP1

p14ARF

Hausp

Mdm2

AKT

MdmxATR

ATM

Wig1

Pirh2

Ub

Ub Ub

Ub

meR

S33

S37

meK

meK

meK

meK

S46

S20

S15p53p53

UbUb

UbUb

Ub

Ub

p53

p53

p53

Strap

Oncogene

Metabolic stress

mRNA

mRNA

Angiogenesis

G2/M transition

Cell cycle arrestSenescence

Cell growthinhibition

Metabolicstress

adaptation

G1/S transition

DNA repair

mTOR

AKT

NADPH/ATPproduction

ROSproduction

Autophagy

Viral infection

DNA damage

Oncogene

Oncogene

Nucleolardisruption

Telomereuncapping

p53 mRNAstabilization

nucleolinL26

p53

p53 mRNAtranslation

p53 mRNAdegradation

Apoptosis

L11

L26

L5

CBP

Ub

UbUbUb

UbUb

p53Proteasomaldegradation

Ub

UbUb

p53

Bax

Bcl-2 Smac

IAPs

CytC

Apaf-1

Casp-9

ROS production

Caspase 9activation

Caspase 8activation

Apoptosis

Mito

p53 pathway

www.abcam.comCopyright © 2011 Abcam, All Rights Reserved.

by Arnaud Vigneron, Andreas Hock, Karen Vousden and Abcam

163-10/10-PW

p53 antibodies from Abcam: See more products at: www.abcam.com

Featured antibodies

p53 [PAb 240] (ab26)

Clonality Applications Host Species cross reactivityM ELISA, Flow Cyt, ICC, ICC/IF, Ms Hu, Ms, Rat

IHC (Methanol fixed), IHC-Fr, IHC-P, IP, WB

ATM [2C1 (1A1)] (ab78)

Clonality Applications Host Species cross reactivityM IHC-P, IP, WB Ms Hu, Ms, Rat, Mk

p21 (ab18209)

Clonality Applications Host Species cross reactivityP ICC/IF, IHC-P, WB Rb Hu

Cdk2 (ab64669)

Clonality Applications Host Species cross reactivityP ICC/IF, WB Rb Hu, Rat

HDAC1 (ab19845)

Clonality Applications Host Species cross reactivityP ICC/IF, IHC-P, WB Rb Hu, Ms, Rat

Product Clonality Applications Host Species Datasheet ProteinsReactivity www.abcam.com/ab…

active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC (PFA fixed), Rb Hu, Ms, Rat, Dm, 13847 52101IHC-FoFr, IHC-Fr, IHC-P, WB InMtj, RMk, Xl, Zfsh

APAF1 M ICC, ICC/IF, IHC-P, WB Rb Hu, Ms 32372 -ATM (phospho S1981) P ICC/IF, WB Rb Hu 79891 -ATR P IF, IP, WB Rb Hu, Ms 2905 -Bax M Flow Cyt, ICC/IF, IP, WB Ms Hu, Ms, Rat 5714 850157Bcl2 P ICC, IHC-Fr, IHC-P, IP, WB Rb Hu, Ms, Rat, Cow 7973 73696BRCA1 (phospho S1423) P WB Rb Hu 90528 -BRCA1 [MS110] M ICC/IF, IHC-Fr, IHC-P, IP, WB Ms Hu 16780 82204BRCA2 P IP, WB Rb Hu, Ms 90541 -Chk1 P WB Goat Hu 2845 -Chk1 (phospho S317) P WB Rb Hu, Ms 2834 -Chk2 (phospho T68) P WB Rb Hu 3501 -Chk2 [2CHK01 (DCS-293)] M IF, IP, WB Ms Hu 3292 -Cdk4 P ELISA, IP, WB Rb Hu, Ms, Rat 6551 56281Cdc14A [DCS-291] M ELISA, ICC, IP, WB Ms Hu 10536 -Cdc25A [DCS-120 + DCS-121] M IHC-Fr, IHC-P, IP, WB Ms Hu, Rat 2357 90763Cyclin D1 [CD1.1] M ELISA, Flow Cyt, IHC-Fr, IHC-P, IP, WB Ms Hu, Ms, Rat 6152 85247Cyclin E [HE12] M Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Ms Hu 3927 -DNA PKcs (phospho S2056) - ChIP Grade P ChIP, Flow Cyt, ICC/IF, WB Rb Hu 18192 -

DNA PKcs (phospho T2609) P ICC/IF, WB Rb Hu 4194 -DNA PKcs [Y393] M ICC/IF, IHC-P, WB Rb Hu, Ms, ArHm 32566 -E2F1 [KH95] M IHC-Fr, IHC-P Ms Hu, Ms, Rat 4070 82207E2F2 P WB Chk Hu, Ms 14292 -E2F4 [SPM179] M GSA, IF, IHC-P, WB Ms Hu, Rat 17845 -E2F5 P WB Rb Hu 22855 -E2F6 [TFE61] M ICC/IF, IP, WB Ms Hu 11952 -HIPK2 P ELISA, ICC/IF, IHC-Fr, IHC-P, WB Rb Hu 28507 -JNK1+JNK2 P WB Rb Hu 85139 -KAT2B / PCAF - ChIP Grade P ChIP, IHC-P, IP, WB Rb Hu, Ms, Dog 12188 82074KAT3B / p300 [3G230] - ChIP Grade M ChIP, ICC/IF, IHC-Fr, IHC-P, IP, WB Ms Hu 14984 82235

MDM2 P ICC/IF, WB Rb Hu 87134 95934MDM2 (phospho S186) [2G2] M WB Ms Hu 22710 -MEK1 P ICC/IF, WB Rb Hu, Ms, Rat 42647 63209MEK1 (phospho T386) P WB Rb Hu, Ms, Rat 5615 -MEK3 P IP, WB Rb Hu 2565 -MEK7 P ICC, IF, WB Rb Hu, Ms 18565 -MEK7 (phospho S271 + T275) P WB Rb Hu 4762 -p38 P ICC, ICC/IF, IHC-Fr, IHC-FrFl, IHC-P, IP, WB Rb Hu, Ms, Rat 7952 45158p53 (phospho S15) P ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rat 1431 -PCNA - Proliferation Marker [PC10] M Flow Cyt, ICC/IF, IHC-Fr, IHC-P, IP, WB Ms Hu, Ms, Rat, Chk, Dm, Zfsh 29 85651PKC gamma P ELISA, ICC, IHC-Fr, WB Rb Rat 4145 60842PKC gamma (phospho T674) P WB Rb Hu, Rat 5797 -Raf1 (phospho S621) P WB Rb Hu 4767 -Raf1 [Y198] M ICC/IF, IHC-P, IP, WB Rb Hu, Ms, Rat 32025 62292SIRT1 P ICC/IF, IP, WB Rb Hu 7343 101130SIRT2 - Nuclear Marker P WB Rb Hu 10659 101132SIRT3 P WB Rb Hu 40963 54333