Post on 24-Feb-2016
description
0 10 20 30 40 500.0
0.20.4
0.6
0.8
1.0
1.21.4
1.6
587 in YP10D + 5mM HMF at 42C
70 mM
60 mM
50 mM
40 mM
30 mM
0 mM
Time, h
OD6
00
0 10 20 30 40 500.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
587 in YP10D+5-HMF at 30C
70 mM
60 mM
50 mM
40 mM
30 mM
0 mM
Time, h
OD6
00
0 10 20 30 40 500.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
587 in YP10D+furfural at 30C
80 mM
70 mM
60 mM
50 mM
40 mM
0 mM
Time, h
OD6
00
0 10 20 30 40 500.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6587 in YP10D+furfural at 42C
80 mM
70 mM
60 mM
50 mM
40 mM
0 mM
Time, h
OD6
00
NCYC587 showed same level of inhibition to furfural, when it was compared with TISTR5925.
NCYC587 showed some tolerance to 5-HMF, when it was compared with TISTR5925.
Attached files for Editor
0 10 20 30 40 50 600.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4 587 in YP10D + Lactic acid (30 ) ℃
0mM200mM300mM400mM500mM600mM
Time (h)
A600
0 10 20 30 40 50 600.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4 587 in YP10D + Lactic acid (42 ) ℃
0mM
200mM
300mM
400mM
500mM
600mM
Time (h)
A600
NCYC587 exhibited the sensitivity to lactic acid, when it was compared with TISTR5925.
Incubation with YPD with acetic acid at 30C
NCYC1429NCYC587NCYC2791TISTR5925
10010-110-210-310-4
NCYC1429NCYC587NCYC2791TISTR5925
10010-110-210-310-4
Kluyveromyces marxianus
10010-110-210-310-4 10010-110-210-310-4
S288cK3K7NCYC3233S3
S288cK3K7NCYC3233S3
Saccharomyces cervisiae
Incubation on YPD with acetic acid at 42C
NCYC1429NCYC587NCYC2791TISTR5925
10010-110-210-310-4
NCYC1429NCYC587NCYC2791TISTR5925
10010-110-210-310-4
10010-110-210-310-4
10010-110-210-310-4
S288cK3K7NCYC3233S3
S288cK3K7NCYC3233S3
Kluyveromyces marxianus TISTR5925 strain has more acid tolerance than other strains. Saccharomyces cerevisiae doesn’t show good performance at high temperature.
Saccharomyces cervisiae