Dr. BEENA BHAT

Executive Vice President, R&D

Sami Labs, Bangalore, INDIA

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NATURE’S BEST – COCONUT

FROM SAMI LABS

WORLD COCONUT DAY

CELEBRATIONS

TNAU, Coimbatore

2nd September 2013

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SAMI LABS / SABINSA

A Research Based Organization

Our Founder

Dr. Muhammed MajeedFounder & MD

Ph.D from St. John’s University

New York

Global Presence

IndiaIndia

ChinaChina

JapanJapan

KoreaKoreaSouth

Africa

South

AfricaAustraliaAustralia

EuropeEuropeAmerica

(NJ & UT)

America

(NJ & UT) VietnamVietnam

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U.S. & International Patents issued: 87

Patent Coverage: India, U.S.A., European Patent Office,

Sweden, Netherlands, Italy, United Kingdom, Ireland,

Luxembourg, Monaco, Portugal, Spain, France, Denmark,

Greece, Switzerland, Belgium, Austria, Germany, Japan,

Australia, New Zealand, Canada and Russia.

Intellectual Property

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U.S. & International Patents Pending : 75

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Our Strengths & Capabilities

- Research oriented company - means constant innovation

and development of new products.

- Capability for New product development as per client

requirements

- Custom manufacturing to customer specifications

- Formulation development lab for nutraceuticals, drugs

and cosmeceutical formulations

- Efficient Manufacturing facilities

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- ISO 22000 and NSF, USA certified facility

- cGMP compliant as per USFDA proposed rules and

approved by FDA, Karnataka for WHO GMP regulations.

- Pilot plant for scaling up of the work done by R&D

- Analytical testing and Biochemical Claim substantiation

capabilities

- In house capability to conduct alternatives to animal

testing

Our Strengths & Capabilities

Over the last twenty five years,

Sabinsa Corporation has pioneered

the manufacture and introduction of

several innovative phytonutrients, to

the US and global markets.

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INCI Name: Cocos Nucifera (Coconut) Fruit JuiceINCI Name: Cocos Nucifera (Coconut) Fruit Juice

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�Coconut water is a liquid

endosperm.

�All nutrients for the embryo

to grow are present in it.

�It is used widely as a

stimulant free energy drink.

From Recent Market Reports…

�New Nutrition Business has just published a

report hailing coconut water as “the fast-

growing new category”, with retail sales

already above $450m worldwide.

�Coconut water is set to redefine the sports

drinks market after Coca-Cola and PepsiCo

both putting their weight behind the new

niche last year.

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Coconut water tipped to redefine sports drink market

www.nutraingredients.com, March 30, 2010

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Actives Reported

�Endogenous hydroxylated aromatic

cytokinins (Topolins and Topolin

ribosides)

�Amino acids

�Vitamins

�Growth factors

Nutrients in Coconut Water

Ref: Contributions from Boyce Thompson Institute, 21:115-128.

Coconut water is rich in proteins,

amino-acids, sugars, vitamins,

minerals and growth hormones

essential to promote tissue growth.

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Organic Alicyclic Acids

�Shikimic acid and Quinic acid are two alicyclic acid that are detected in maximum amounts in water from young green Coconuts.

�The probable role of these acid in the aromatic biosynthesis indicate their potential role in the development of the coconut.

Contributions from Boyce Thompson Institute, 21:115-128.

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The importance of Minerals

Several important minerals

Major (mm/l), - Ca,K,Mg,Na, …

Minor (µm/l), - B,Mn,Zn…….

Trace quantities (nm /l) – Al, Cu,Mo….

are found in coconut water

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Coconut water solids would also provide a nutritive medium for the beneficial microflora in the gastrointestinal tract.

A recent report also mentions the successful use of coconut water as a short-term intravenous hydration fluid.

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Nutritional Benefits

� A refreshing beverage,

� A natural rehydration medium,

�Used as a supplement in nutrient media for tissue culture.

�Natural isotonic, hypoallergenic, sports drink

�Offers higher amounts of electrolytes such as potassium and magnesium than regular sports drinks

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Nutritional Benefits… contd….

� As a prebiotic, coconut water solids support

beneficial bacteria in the gut.

� Provides essential nutrients - proteins, amino acids,

sugars, vitamins, minerals and growth factors.

�Natural enzymes present in coconut water support

digestion, and elimination of toxic waste.

� Reported to support normal blood pressure levels

Alleyne, T et al. West Indian Med J. 2005 Jan;54(1):3-8.

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Coconut water is described as an isotonic sports

drink.

Mineral contents of Coconut water are given below

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RNA-P/DNA-P RATIOThe RNA-phosphorus (RNA-P) content of coconut water was found to be

consistently high.

The role of RNA in amino acid transport and respiratory metabolism of

living cells is well known.

Contributions from Boyce Thompson Institute, 21:115-128.

Coconut water works at cellular level

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Cytokinins are a class of plant hormones.

When cytokinins are present, the cells can then replicate

to form a perfect copy of its DNA.

If they are not present, imperfect cells are formed

resulting in future chronic illnesses.

Coconut water contains concentrated amounts of these

Cytokinins.

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A major cytokinin found in coconut milk was

isolated using a standard procedure, the

tobacco callus growth promoting assay. The

structure was determined to be a complex

trans-zeatin riboside (G3A2-ZR).

The authors of this study attributed at least

20% of the cytokinin activity in coconut milk

to this compound.

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Cytokinins is a class of plant growth

substances (plant hormones) active in

promoting cell division.

They are also involved in cell growth and

differentiation and other physiological

processes.

• Sami Labs Limited has pioneered the

successful commercialization of several

natural innovative products from Coconut.

• Coconut became an important source for

four of our products

- Lauriforte®

- Cococin ®

- MonoLaurin

- CocoProteins - under development

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Sami Labs has been granted

�European Patent No. EP 1341547

�US Patent No.7,300,682

�US Patent No.8,247,003 (Cococin®+ Saberry®)

�US Patent No.8,329,095 (Composition containing

Monolaurin)

Intellectual Property

US

7,300,682EP

1,341,547

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Lauriforte ®

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The genesis of Lauriforte®

Coconut oil is found to have a lot of beneficial

effects on the skin and body.

The main issue was its cosmeceutical non-

acceptance due to its poor drying and greasy

nature.

With focused research, Sami Labs, overcame

these problems with its Lauriforte®.

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Lauriforte®

Lauriforte® is the registered trademark of

Sabinsa Group .

It is the Lauric Acid enriched fraction of

Coconut Oil.

It is non-greasy with versatile applications in

cosmetics.

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Lauriforte ® – The Hair Care

Cosmeceutical

�Prevents combing damage to various hair types.

�Provides nourishment to the hair.

�Has excellent penetration to the hair shaft and

follicles.

�Provides protection against hygral fatigue of the

hair.

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The Nourishment factor®The Nourishment factor®

A Major Nutraceutical and Cosmeceutical IngredientA Major Nutraceutical and Cosmeceutical Ingredient

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Cococin®� Sami Labs has developed a research based product

from coconut.

� Cococin® is tender coconut water concentrated under

carefully controlled conditions.

� It is available as a free- flowing powder or a syrupy

liquid.

� Cococin® is rich in carbohydrates, amino acids and

natural salts.

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Cococin® Composition

Two Probable Mechanisms Of Cell Growth

Promotion By Cococin™

Coconut water is used as a nutrient medium in plant

tissue culture.

Several inherent constituents like Organic Alycyclic

Acids, high RNA phosphorus content & Cytokinins are

reported to be responsible for the growth promoting

actions.

Freeze-drying under controlled conditions ensures that

the solids retain optimum biological activity.

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Clinical Study Age Defying Complex

� 22 healthy female volunteers, in the age group 20-35 years.

� Cream base containing 1% Cococin (& other natural ingredients) was applied on the left arm, and cream base was applied to the right arm.

� Duration: 8 weeks.

� Parameters Measured:

%reduction in skin roughness parameters

% reduction in skin elasticity

� The skin roughness was measured using the Skin Visiometer .

� The skin elasticity was measured using the Cutometer.

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Comparative effects of Test Cream (Test) and

Cream Base (placebo) on skin elasticity

-5

0

5

10

15

20

25

30

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30 Days 60 Days

24.58

31.19

13.74

-2.38

% Change in

Elasticity

Time Test Placebo

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Wound Healing and Fibroblast

� The arrival of fibroblasts cells into the wound, heralds the healing process.

� Eventually, these Fibroblasts become the dominant cell type, during healing process.

� As the Fibroblasts proliferate, the keratinocyte and endothelial cell populations are also stimulated to increase their numbers.

� Thus an active, which promotes the proliferation of Fibroblasts has good wound healing potential.

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Cell Proliferation enhancement of Cococin® in

Swiss 3T3 fibroblast cell line

Control day 1 Control day 2

Cococin day 1Cococin day 2

Study Report by Sami Labs Limited

Cococin® vs Foetal bovine serum

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Cococin™ was shown to promote the proliferation of fibroblasts and hence can be

inferred to have good wound healing potential.

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In view of the role of coconut water

solids in supporting cell growth,

Cococin® may be used in applications

to support the growth of human

tissues such as hair follicles.

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The product may therefore be used

in hair care formulations and in

rejuvenative topical preparations.

Cococin®, a Natural cell-growth promoter has

been evaluated for its efficacy as a hair

growth promoter, in comparison with

Minoxidil as standard.

The study was conducted at Dabur Research

Foundation, New Delhi.

Cococin® in Hair growth

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Cococin ™ in Hair Growth

�Hair growth promoters are known to

potentiate the transition of Telogen phase

(Shedding Phase) to Anagen phase (Growth

Phase).

�This is manifested by increase in follicle count

in subcutis, increase in skin thickness and

graying of the pink skin.

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Treatment Mean follicle count

in subcutis layer

Average skin

thickness (µm)

% of animals showing

Anagen induction

Cococin ™ Cream

(2% Cococin)

43.00 ± 12.20 220.15 ± 25.60

71.4

2% Minoxidil 42.86 ± 13.49 218.75 ± 34.04 71.4

5% Dextrose 3.43 ± 3.27 156.27 ± 5.14 14.2

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Cococin® in Hair Growth

Both 2% Minoxidil

and 2% Cococin®

showed Telogen to

Anagen Phase

transition in more

than 70% of the

treated animals.

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Cococin® was found to be safe for topical

use.

In laboratory studies, the material was

found to have zero skin irritation potential

and LD50 values greater than 2000 mg/kg

when applied on the skin.

Safety Studies

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ANEW CLINICAL Deep Crease Concentrate with Bo-Hylurox™

&

ANEW LUMINOSITY ULTRA advanced skin brightener SPF15

UVA/UVB

Cococin® has been used in

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Cosmetic products in which Cococin® are

included

Anti-ageing 8 formulations

Skin moisturizer 1 formulation

Wound healing 3 formulations

Hair Care

(Hair growth promotion)

1 formulation

Improved probiotic 1 formulation

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MonoLaurin

Image: readanddigest.com

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MonoLaurin

• MCTs are well recognized for their antimicrobial properties since antiquity.

• Lauric acid, a MCT present in good concentrations in coconuts, forms MonoLaurin in the body.

• MonoLaurin is reported to significantly inhibit the growth of pathogenic microbes.

• In vitro, MonoLaurin’s effects mirrored Origanum oil in inhibiting S. aureus.

MCT content in Coconut

• Fresh coconut / unrefined or Virgin Coconut oil is highly

saturated; over 85 - 90% of its fat content is saturated.

• Saturated fatty acids may be (a) short chain – 2, 3 or 4 carbon

atoms (b) medium chain – 6, 8, 10 or 12 carbon atoms (c) long

chain – 16 or more carbon atoms.

• About 60 - 70% of the fatty acids in coconut oil are 12 carbon

atoms or shorter, thus making it a rich source of MCTs.

• Coconut oil is high in lauric acid (12 carbon atoms) and contains

trace amounts of caprylic acid (8 carbon atoms).

Gopala Krishna A G etal., (2010) Indian Coconut Journal. 15-27.University of Minnesota, 2005. NutriNet

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MCTs in Coconut

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Lauric acid

Caprylic acid

Benefits of Coconut MCTs

Coconut medium chain fatty acids can have beneficial roles to play

in human health.

• Reduce body fat mass by inhibiting the expression of fat-

generating genes in nuclear DNA.

• Increase the sensitivity of body tissues to insulin. This effect can

help address the problem of the pre-diabetic state known as

Syndrome X or Metabolic Syndrome.

• Boost a person’s overall energy level.

• Increase whole body metabolic rate.

• Support a weight management regimen.

• Ability to provide the brain with an alternate source of energy

http://www.omegascience.org/product_ingredients/coconut_oil.aspx

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Due to their longstanding safety record,

Origanum and/or MonoLaurin, alone or

combined with antibiotics,

“might prove useful in the prevention and

treatment of severe bacterial infections,

especially those that are difficult to treat

and/or are antibiotic resistant.”

Effects of Essential Oils and Monolaurin on Staphylococcus aureus: In Vitro and In Vivo Studies Harry G. Preuss et

al., Department of Physiology and Biophysics, Georgetown University Medical Center, Washington, D.C. 20057

MonoLaurin

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Our well known international customers have evinced great interest in this coconut product for its health providing and cosmetic applications.

END USER SATISFACTION

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Sami Labs is interested in pursuing

1. Basic Research

Identifying and isolating several actives from Cococin® and characterizing them.

The actives may be in the form of small proteins, peptides and enzymes, growth factors.

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2. Product Research

Developing cosmetic products and bringing

them to international market with complete

efficacy studies, total safety testing and

validated analytical method and human

clinical trials.

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3. Technologies for manufacture

� Use of membrane filters (Pennwalt Technology) to remove oils/fats.

� Partial concentration using Reverse Osmosis (RO) technology.

� Lyophilisation on a commercial scale.

� CENTRITHERM - New equipment from Australia.

Coconut Water Concentration-use

of “CENTRITHERM”

“CENTRITHERM” is a state of art equipment developed

and patented by an Australian company named

“FLAVOURTECH”

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“CENTRITHERM” is a highly specialized

equipment capable of concentrating

aqueous (water based) solutions at low

temperatures which preserves the natural

integrity of the juices, water extracts of

fruits, vegetables, berries etc.

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SAMI LABS is planning to buy the smaller

version costing about 1.3 crores.

With additional requirements on utilities/

facilities for collection of coconut water in

sterile condition the total Project cost would

be about 1.6 crores.

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THANK YOU

www.sabinsa.com participating members of:

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