Post on 17-Aug-2020
Making Measurements using
Fiber Optics and the Cary 60
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MARK FISHER APPLICATIONS ENGINEER
AGILENT TECHNOLOGIES
21 May 2015
Applications for which Fiber Optics might be Ideal
Working Solution
• In Situ Measurements (kinetics)
• Working with small volumes
• Can not bring Sample to Instrument (Art Restoration)
• Desire to reduce transfer of Samples to Cuvettes
• Measurement Needs to be made a distance from Instrument
• Measurements need to be made in an inert atmosphere
(glove box)
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Cary 100
UV-Vis NIR Spectroscopy Solutions
The Agilent Cary Family
Cary 300
Cary 4000
8453
Cary 5000/6000i
Cary 60
Cary
Eclipse
Perf
orm
ance
Routine to Research
“A word from our sponsors”
What are Fiber Optics
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Fiber Optics
Cladding
Central Core
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How Does Optical Spectroscopy Work From The
Instrument View
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Source Energy
Selection Sample Detector Display
Why the Cary 60 ?
Cary 60 is uniquely designed :
• Small beam spot size
• Room Light Immunity
• Short Pulse Duration (key for photosensitive materials)
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Regular Cuvette Holder for Cary 60
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Beam Size as It Enters Sample Compartment
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Beam Size at Focus in Middle of Cell Holder
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Fiber Optic Couplers and Immersion Probes
Fiber Optic Couplers for the Cary 60
Immersion Fiber Probe Design
Immersion Fiber Probe Replaceable Tips (reusable)
Stainless Steel with replacement tips
Torlon Probe with replaceable tips
Quartz Probe (does not come with replaceable tips)
Versa Probe with Disposable Tips (NOT reusable)
Micro Probe (does not come with replaceable tips)
Fiber Optic Couplers for Cary 60
The Fiber Optic Dip Probe is a
trouble-free alternative to a
conventional sipper.
The Fiber Optic Coupler allows
you to connect a fiber optic
probe to suit your application.
Immersion Fiber Probe Design
• Stainless Steel Probe
• Torlon Probe
• Quartz Probe
• Micro Probe
• Versa Probe
Immersion Fiber Probe Replacement Tips
(reusable)
Replaceable Tips come in a
various selection of path lengths
and are cleanable and reusable.
These tips can be used on the
Stainless Steel and Torlon
Immersion Probes.
Cary 60 Differentiators
Fiber Optics No more need for expensive cuvettes!
Improve workflow by taking the instrument to the sample
Measure cold, hot and odd-shaped samples
Measure
cold
samples
directly from
the
refrigerator!
Improve
workflow
and
minimize
sample
preparation
APPLICATION NOTES AVAILABLE:
5990-7863EN Measuring purity of low volumes of DNA at 4 ºC with fiber optics microprobe
5990-7945EN Determination of drug tablet concentration in pharmaceutical applications for drug development
5990-7864EN Simple, automated measurements of the photocatalytic properties of colorimetric species
Application Focus
No compromise in data quality,
strong linear correlation:
relationship between
absorbance and concentration
Abs
DNA concentration (ng/µl)
y = 0.0198x R² = 0.9996
0.0
0.5
1.0
1.5
2.0
0 20 40 60 80 100
DNA scans using fiber
optic microprobe
www.agilent.com (Publication Number 5990-7863EN)
Short Pathlength Solution using Fiber Optics
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• Easy to clean and load
• No sample carry over
• Recovery of precious samples
• Different path length options
• Can be thermostated
• Ease of use:
• Pipette sample
• Place lid over sample
• Take measurement
• Wipe clean
Tray Cell Performance Benefits
Measure High Abs With Short Paths
Tray Cell Accuracy Cary 4000 vs Cary 60
Hellma TrayCell – linearity
20
Excellent linear
performance to 2.3abs
Detect down to 0.001
mg/mL of BSA (using 10mm
pathlength cuvette)
Dedicated software
Hellma TrayCell
10mm cuvette 1.0mm cap 0.2mm cap
max abs 3.3 abs 2.3 abs 2.3 abs
dilution factor 10x 50x
equiv abs 23 abs 115 abs
No dilution necessary 0 to 115 abs!
More Options for Measurements of Microvolumes
by UV-Vis
For customers who need to measure the concentration or purity of small volumes
of precious samples such as nucleic acids or proteins - there is a better
alternative! With a design focused on ease of use, and superior performance in
sensitivity and reliability, the Agilent solution is the Cary 60 and the ultra-
microvolume cuvette accessory, which now has more options available for a
wider range of sample volumes and concentrations.
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Cary 60 With Microscope
Measurements made in a Inert Atmosphere
(glovebox)
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Cary 60 with Fiber Optic Coupler
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Fiber Optics to the Glovebox Feed-through
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Glovebox Feed-Through
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Inside Glovebox
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Cuvette Holder Inside Glovebox
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Sample Measurement Outside Glovebox
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Diffuse Reflectance Measurements using Fiber
Optics
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Cary 50 With Remote DRA and Camera
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Useful in Art Restoration Projects
• Allows measurement to be made at painting
• Mount Accessory on an X-Y stage
• Able to fix distance from art work to accessory
• Use Color software to determine L*a*b or other color values
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Fiberoptic Dissolution
Options with the Cary 60
Fiber Optic Benefits vs. Multicell Systems
• Very fast sampling times,
30s+
• More time points ideal for
early formulation work and
immediate release
formulations
• Able to analyze samples
where filtration is not
possible/practical (<0.45um)
• Can work in extremely low
volumes
• No Consumables
Why Fiber Optics on the Cary 60?
• Highest optical transmission of any system on the market allowing it to easily read in high turbidity solutions
• Can lose over 90% transmission and still have more transmission than other fiber optic systems on the market
• Flashlamp lamp preserves life of fiber optic probes
Agilent’s New Technology – Fiber Optic Probes.
• Specially designed for dissolution testing.
• 600 micron silica/silica Fibers for optimized transmission down to 190 nm.
• 1, 2, 5, 10, 20mm tips available.
• Streamlined to minimize hydrodynamic influence.
Fiber Optics not just for UV-Vis Measurements
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Cary Eclipse Fluorescence Spectrometer
The Power of Xenon…
• Unique Xe flashlamp technology
• Measure small volume samples
• Fiber optics
• Room light immunity: unique,
Varian patented technology
• Eliminates photo-degradation
• Long lamp lifetime
Application focus
• Biochemical applications
• Academia
• Industrial chemistry
Special Thanks
Special Thanks to Dr. Jillian L. Dempsey with the Department of
Chemistry at the University of North Carolina and her graduate
student, Robin Knauf, for providing access to their Cary 60 and
glove box as well as a sample they are interested in for use in
data collection for this presentation
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Thanks For Your Patience And Good Humor
Shortly You Will Receive A Link
To Down Load The Presentation
My Contact is Mark.Fisher@Agilent.com
Thanks Again