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Lecture 6: Molecular Techniques

1. Restriction Mapping2. Molecular cloning

Inserts and vectors

Restriction enzymes and ligaseFig. 9., 9.5b, 9.6; 9.7, 9.8, 9.9, 9.10,9.11, 9.12Table 9.1, 9.2Read p279-293

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Fig. 9.2

Fig. 9..2

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Fig. 9.6

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Fig. 9.5b

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Gel electrophoresis

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2. Molecular CloningInserts and vectors

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Restriction Digestion

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Fig. 9.7

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Fig. 9.8

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Fig. 9.9

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Genomic LibraryHuman: 3,000,000 kb/haploid genome150 kb per insert--20,000 clones equals a genome10kb per insert--300,000 clones (genome equivalent)

cDNA library

Expression Libraries

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Fig. 9.10

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Fig. 9.11