Kyung Jae Jeong, Ph.D., Claes H. Dohlman, M.D., Ph.D., and Daniel S. Kohane, M.D.,

Ph.D.Harvard-MIT Health Sciences and Technology,

Department of Anesthesiology, Children’s Hospital Boston, Harvard Medical SchoolMassachusetts Eye and Ear Infirmary, Harvard Medical School

*The authors have no financial interest in the subject matter of this poster.

Khan et al. (2008)

Cell migration Cell proliferation with minimal

immune response ECM Production Adhesion

Good biointegration will also reduce the danger of bacterial/fungal infection.

Dopamine (or DOPA) is a major component of mussel adhesive protein and has good adhesive properties.

Polydopamine coating can form on virtually all surfaces.

Polydopamine coating can further be used to immobilize biological molecules which contain either thiol or amine groups at a high density.

Chemistry is very simple.

Lee et al. Nature (2007)

Lee et al. Adv. Materials (2009)

PMMA PMMA-RGD

Dopa-PMMA Dopa-PMMA-RGD

MTS assay on corneal epithelial cells

Fluorescence micrographs of epithelial cells. Nucleus (blue) and actin cytoskeleton (red)

PMMA PMMA-RGD

Dopa-PMMA Dopa-PMMA-RGD

MTS assay on keratocytes

Polydopamine coating does not release dopamine monomer (mass spectrometry)

Minimal contraction of the aortic smooth muscle cells (Calcium Flux Assay). Not significantly high level of proinflammatory cytokines released from the

cells seeded on the polydopamine surface (ELISA, IL-6 and TNF-α).

1400

1200

1000

800

600

400

200

0

Con

cent

ratio

n o

f IL

-6 (

pg/m

L)

PMMA PMMA-RGD Dp Dp+RGD TCP LPS

* *

**

*

****

* *

Keratocytes Epithelial Cells

Calcium assay. on human aortic smooth muscle cells (HASMC). Red point corresponds to polydopamine coating. Higher fluorescence response corresponds to higher cell contraction.

IL-6 concentration from the cells seeded on various surfaces after 24 hours. TNF- α was not detected from neither cell types.

PMMA PMMA-RGD

Dopa-PMMA Dopa-RGD-PMMA

PMMA Dopa-PMMA

A small PMMA cylinder was pressed onto collagen gel over night to see if there is any adhesion at the interface.

Collagen Gel

PMMA Cylinder

Polydopamine coating enhanced the proliferation of corneal epithelial cells and keratocytes in vitro.

Polydopamine coating is not proinflammatory and is not expected to cause vascular constriction as its monomer would do.

SEM images show ECM secretion from all surfaces. Both polydopamine-coated surfaces exhibited firm

adhesion to collagen gel.

Polydopamine coating may enhance the biointegration of Boston KPro when applied to the stem of the device.