Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①.

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Transcript of Introduction and lab Rules Weiwei TANG adatong2007@126.com Biochemistry Experiment ①.

Introduction and lab Rules

Weiwei TANG

adatong2007@126.com

Biochemistry Experiment ①

2 |Biochemistry laboratory

1. Aims of Biochemistry Experiments

Experiments Rules

Instruments Introduction

GRADING

Theory EXAM : 70%Attendance(Theory): 10%Lab Performance: 20%

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2. Experiment Rules

① Put on your white lab coat before the class begin

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2. Experiment Rules

② It is better done than said

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2. Experiment Rules

③ Make Compensation

For Any Damage

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2. Experiment Rules

④ Please shut off

the faucet and Power

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2. Experiment Rules

⑤ Clean the classroom before you leave!

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If you break any rules above, I will lower your 

participation  grade !

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3. Instruments Introduction

① check instruments for each group by the table below

category number category number

250-ml beaker 1 Dropper 1

100-ml beaker 1 Glass stirring rod 1

Large Test tube 18 Test-tube rack 1

Small Test tube 6 Bulb for pipette 1

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3. Instruments Introduction

Beaker

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3. Instruments Introduction

Test tube

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Solution Mixing

Rotation

Fliping

Shaking

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3. Instruments Introduction

Test-tube

rack

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3. Instruments Introduction

Test-tube

brush

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Test tube Washing

1. Flush the test tube with water At least 3 times

2. Scrub it with  detergent both inside & outside

3. Flush the test tube with water at least 5 times just like step1

4. Rinse it with distilled water and place

them upside down on the rack to leave it dry

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3. Instruments Introduction

Dropper

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3. Instruments Introduction

Glass-stirring rod

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3. Instruments Introduction

Tube clip

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3. Instruments Introduction

Bulb for pipette

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3. Instruments Introduction

Scaled pipette

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Scaled Pipette Pattern:

0.5 ml 、 1 ml 、 2 ml

5 ml 、 10 ml etc.

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• Hold Like This

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Usage

Hold pipette

Absorb Solution

Adjust the Volume

Drop Solution

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3. Instruments Introduction

Micropipette

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Micropipette Specifications: 10 μl 、 20 μl 、 100 μl 、 200 μl 、 1000 μl & 5000 μl

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THE INTRODUCTION ON PIPETTE

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THE INTRODUCTION ON PIPETTE

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Usage

1. Set the volume of solution

2. Choose paired tip ,and put on it

3. Absorb solution with 1st Lock , drop solution with 2nd Lock.

Summary

1. Be careful

2. Be patient

3. Be lively

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Please hand in your Report in time !

Thank youBiochemistry laboratory

Changsha Medical University

LAB REPORT

LAB REPORT

LAB REPORT