Post on 04-Jan-2016
Improvement of Rainfed Lowland Rice Variety for Cooking Quality Traits using MAS
Sureeporn KatengamUraiwan Kotchasatit
UBONRATCHATHANI, THAILAND
Background Rice production in NE of Thailand Area of production ranging from
drought-prone lands to flooding area
Abiotic stresses related with unpredictable rainfall pattern are main problems
State of problem Abiotic stresses associated with
unpredictable rainfall pattern are main problems for rice production in NE of Thailand.
Elite cultivars resistance to drought are required.
State of problem (cont.) KDML105, an aromatic rice with good
cooking quality, is widely grown in NE, however, its production is dominating constraint with the drought problem.
Drought tolerance is a complex traits since this trait is governed by many QTLs locating in many chromosome (ch1, ch2, ch3, ch4, ch7, ch8,and ch9).
Therefore, improvement KDML105 for drought resistance is not an easy tasks.
State of problem (cont.) In the opposite way, improving of well
adapted variety to drought prone areas for other quality traits such as cooking quality is worth to reconsider.
Cooking quality trait is determined by three characters specifying the physical properties of starch in the endosperm:
Amylose content (AC) Gel consistency (GC) Gelatinization temperature (GT)
Objectives Marker-assisted backcross breeding is
aimed at improving well drought adapted variety to rainfed lowland area of NE of Thailand for cooking quality traits utilizing tightly linked DNA markers.
The major QTL controlling AC, GC, and GT are located on Ch.6. DNA marker of flanking markers closely linked to this major QTL controlling quality traits will be carried out for MAS.
Plant materials Donor parent:
KDML105 low AC (~16-17%): major QTL on ch6 (at Wx
gene ) low GT: major QTL on ch6 medium GC: major QTL on ch6 (at Wx gene )
Recipient parent: IR57514-drought
Breeding scheme:
Chr.6 Chr.11
Map location of QTLs controlling cooking quality traits
Genetic map (Azu / IR64 )
# Markers Traits ChromKDML 105 IR57514 KD571-77Size expected Remark1 BADH Aroma 8 ~ 400 ~410 ~400 Polymorphic
2 RM190 Amylose content 6 ~120 ~116 ~120 124 Polymorphic
3 Waxy & Gel consistency 6 ~311 ~315 ~311 Polymorphic
4 RM204 6 ~155 ~119 ~155 169 Polymorphic
5 RM205 6 121 121 121 Monomorphic
6 RM225 6 ~145 ~120 ~145 140 Polymorphic7 RM111 Gelatinization 6 120 120 120 124 Monomorphic
8 RM50 temperature 6 200 200 200 201 Monomorphic
9 RM121 6 ~275 ~272 ~275 Polymorphic
10 PSS-1-1 6 149 149 149 Monomorphic
11 PSS2-2 6 200 no 200
12 RM136 6 290 290 290 101 Monomorphic
13 GT11 6 ~200 ~195 ~200 Polymorphic
14 RM229 11 ~119 ~119 200 116 ?
15 RM457 11 ~230 ~232 ~230 230 Polymorphic
16 RM21 11 no no no 157 ?
17 RM473E 11 141, 139 122,119,118 118 Multiple bands
117 117,116 (repeat)
18 RM206 11 ~160 ~148 ~120 147 ? (repeat)
19 RM254 11 ~156 ~156 ~153 165 ?
20 PB7-8 Bacterial leaf blight(Xa21) 11 no no no ? (repeat)21 RBO 783 Submergence tolerance 9 375 ~350 ~350 Monomorphic
Expected size (bp) Survey of SSR markers for parent screening
1 KD2 IR575143 KD571-77
1 2 3R
M59
7R
M19
0
RM
204
RM
205
RM
225
RM
111
RM
50
RM
121
RM
136
GT
11
RM
229
RM
457
RM
473E
RM
206
RM
254
RM
21
PS
S II
-1
PS
S II
-2
BA
DH
WA
XY
RM
21
RM
121
726bp
553bp500bp
427bp
311bp
249bp
200bp
151bp140bp
118bp
100bp
PCR condition
Thermal cycling
Screening parents with SSR markers
# Markers Traits ChromIR57177KD571-77Expected size (bp) Remark
1 BADH Aroma 8 ~410 ~400
2 RM190 Amylose content 6 ~116 ~120 124
3 Waxy & Gel consistency 6 ~312 ~311
4 RM204 6 ~119 ~155 169
5 GT11 Gelatinization 6 ~195 ~200
6 PSS2-2 temperature 11 Agarose
7 RM457 11 ~232 ~230 230
8 RM21 11 157 repeat
9 RM206 11 147 repeat
10 PB7-8 Bacterial leaf blight(Xa21) 11 Agarose
SSR markers chosen for parents screening
donor recipient KD571-77 X IR57514
2nd workshop on DecemberMAS for BC1F1
F1
F1 X IR57514
BC1F1
mid-October
mid-July
late-June
Planting parents
mid-November
Planting F1 and recipient parents
1 2 3 4………………………………. .200 lines
Line selection with selected SSR markers
early-April
early-August
late -November
MAS