Post on 13-Dec-2015
http://tums.ac.ir/faculties/emaneini
Mohammad Emaneini,
PhD
Assistant Professor in Microbiology
Department of Microbiology
School of Medicine
Tehran University of Medical Sciences
emaneini@tums.ac.ir
Emaneini M. PhD.
Transformation A DNA fragments/plasmids of donor bacteria
Taken up & incorporated into the genome of recipient cell
Natural competence
Streptococcus pneumoniae, Haemophilus influenzae, Neisseria
gonorrhoea
Induced competence
Cold CaCl2 & heat shock: E. coli
Protoplast: Bacillus, Streptomyces
Electroporation: a brief pulse of high-voltage electricity
Emaneini M. PhD.
TransductionTransfer of DNA by Bacteriophage via Lytic & Lysogenic cycle
Generalized transduction Specialised transduction
Emaneini M. PhD.
Discovered 1946 Lederberg &
Tatum
Donor:
Containing conjugative plasmid
(F+)
Recipient:
Bacterial receiving the plasmid
(F-)
Conjugation
High-frequency recombination (Hfr)