Post on 22-May-2015
HISTOLOGY SLIDES
FIRST YEAR MBBSRawalpindi Medical College
SIMPLE SQUAMOUS EPITHELIUMIdentification points:• Flattened cells• Surface view: rounded central nucleus• Cross section: elongated nucleus
SIMPLE CUBOIDAL EPITHELIUMIdentification Points:• Cuboidal cells (height almost the same as width)• Central rounded nuclei• Surface view: polygonal
SIMPLE COLUMNAR EPITHELIUMIdentification points:• Single layer of elongated cells• Height of cell > width of cell• Elongated basal nuclei
SIMPLE COLUMNAR EPITHELIUM WITH GOBLET CELLS
Identification Points:• Elongated column shaped cells (height > width)• Elongated basal nuclei• Goblet cells have central and oval nuclei
STRATIFIED CUBOIDAL EPITHELIUMIdentification points:• Multilayered• Basal cells: high cuboidal or low columnar• Surface layer: cuboidal
STRATIFIED SQUAMOUS NON KERATINIZED EPITHELIUM
Identification Points:• Surface squamous flattened cells• No keratin• Middle layer: polygonal cells• Basal layer: high cuboidal/low columnar cells
PSEUDO STRATIFIED COLUMNAR EPITHELIUM
Identification Points:• Single layer of cells• Some cells are columnar; others are cuboidal• Nuclei lying at different levels; give the false appearance of ‘stratified’
epithelium
EPIDIDYMUS
TRANSITIONAL EPITHELIUMIdentification Points:• Basal layer of cuboidal cells• Next, four layers of polygonal cells• Next, two layers of pear shaped cells• Upper most layer of umbrella shaped cells
THIN SKIN
Identification Points:• Stratified squamous keratinized epithelium• Dense irregular CT beneath• Hair follicles and roots present• Sebaceous glands• Sweat glands
THICK SKINIdentification Points:• Stratified squamous keratinized epithelium• No hair roots and follicles• Pacinian corpuscles
DENSE REGULAR C.T
Identification Points:• Parallel collagen fibres in the form of bundles• Compressed elongated fibroblasts between the collagen fibres
MUCOUS ACINIIdentification Points:• Pyramidal cells• Apical portion lightly stained• Basal portion darkly stained• Flattened nuclei towards the base
FIBRO CARTILAGE
Identification Points:• No perichondrium• Chondrocytes within lacunae, arranged in rows• Collagen fibres arranged parallel
ELASTIC CARTILAGE
Identification Points:• Perichondrium is present• Elastic fibres in matrix• Basophilic ground substance around lacunae• Chondrocytes in lacunae in the form of isogenous groups
HYALINE CARTILAGEIdentification Points:• Dense Irregular CT (perichondrium)• Chondrocytes within lacunae• Chondrocytes in the form of isogenous groups• Naked eye examination: Bluish white translucent appearance
Costal Hyaline cartilage
ARTICULAR CARTILAGEIdentification Points:• Superficial tangential layer• Intermediate transitional layer• Radial zone• Calcified zone
COMPACT BONEIdentification Points:• Haversion Canal• Lamellae• Osteocytes in lacunae• Volkmann’s canal
SPONGY BONE
Identification Points:• Trabeculae• Red marrow• Spikes• Periosteum: outer fibrous, inner cellular
CARDIAC MUSCLE
Identification Points:• Branched• Striated• Intercalated discs
SMOOTH MUSCLEIdentification points:• Non striated appearance• Single central nucleus• Spindle shaped cells
SKELETAL MUSCLES
Identification Points:• Characteristic striated appearance• Peripheral nuclei• Nucleus ovoid in shape
PERIPHERAL NERVE
CEREBRUMIdentification points:• Pyramidal and non pyramidal cells• Arranged in the form of layers (mention the names) – properly visible only
by ‘Nissl staining method’
CEREBELLUM
Identification Points:From outwards to inwards:• Molecular layer with fan shaped dendrites, stellate cells and basket cells
(but cell population is very low)• Flask shaped Perkinge cells• Granular layer (deeply basophilic appearance of closely packed nuclei)
SPINAL CORDIdentification Points:• Central canal• Butterfly shaped grey matter• Dorsal median sulcus and ventral median fissure (depending on the
portion under examination)
ELASTIC ARTERY
Identification Points:• No distinct outer and inner elastic membranes• Tunica adventitia: merges with CT, contains vasa vasorum• Tunica media: elastic fibres are present in a large amount• Tunica intima: Simple squamous endothelium, subendothelial CT
MUSCULAR ARTERYIdentification Points:• Tunica intima: darkly stained• Tunica media: smooth muscle fibres arranged in circular patterns• Tunica adventitia: collagen (lightly stained); elastic (darkly stained)
LYMPH NODEIdentification Points:• Primary follicles/nodules without germinal centre• Secondary follicles/nodules with germinal centre• Irregular anastomosing ‘medullary cords’• Subcapsular sinuses
THYMUSIdentification Points:• Medulla is lightly stained• Hassall’s corpuscles in medulla• Epithelioreticular cells; stellate shaped
SPLEENIdentification Points:• Red pulp: splenic cord and venous sinuses• White pulp: contains lymphatic nodules and germinal centre• Splenic sinuses• No differentiation into cortex and medulla
TONSILIdentification Points:• Stratified squamous non keratinized epithelium• Crypt• Trabeculae• Lymphatic nodules
TRACHEA
LUNG
Identification Points:• Alveoli: Type I alveolar cells (simple squamous epithelium); Type II alveolar
cells (cuboidal cells)• Respiratory bronchioles: lined by simple cuboidal epithelium
ADIPOSE TISSUE(WHITE)
Identification Points:• Unilocular• Nuclei towards periphery• Spherical
THE END
By Nowera Zafar