Histology Lab 1

Introduction and Epithelium

Jun Zhou（周俊） , Ph.D & M.DSchool of Medicine,Zhejiang University

Note:Please come lab on time.Find your seat according your lab number.

Your final score is composed of four parts 1) Attendance and picture drawing:10% 2) Quiz: 15% (each quiz 5%) 3) Final lab test: 25% 4) Final written examination: 50%

How to pass this course: Final score>60 and Final written exam>50

Lab facilities: 1 Light electron microscope with computer 1 case of slides 1 atlas http://m-learning.zju.edu.cn 1.Students share these facilities2.Do not take them out the laboratory with you.3.Put them where they belong to when lab is over.4.Do not left any rubbish after you.5.No food in the lab.6.Lecture and lab slides online: m-learning.zju.edu.cn 2015 Histology and Embryology

Contents of Lab 1:1. Introduction

2. Epithelium

1)Simple squamous epi (small intestine,stomach)

2)Simple cuboidal epi.(thyroid)

3)Simple columnar epi.(small intestine)(drawing)

4)Pseudostratified ciliated columnar epi.(trachea)

5)Stratified squamous epi.(esophagus)

6) Transitional epi.(bladder)

3.Lab report (content of lab , drawing)

Tissue ProcessingTissue Processing

1.Fixation: 10% Formalin

2.Dehydration:graded concentrated ethyl alcohol

3.Clearing:xylene,infiltrate with paraffin solvent.

Loss of fat due to ‘clearing’

Tissue ProcessingTissue Processing

4.Embedding4.Embedding

5.Sectioning5.Sectioning

Tissue ProcessingTissue Processing6.Staining6.Staining

Common Stains: Hematoxylin & EosinCommon Stains: Hematoxylin & Eosin

Ross, 5th ed, fig 1.1

Common StainsCommon Stains

Wright’s stain

Hematoxylin and eosin ("H&E")

Relative Sizes in HistologyRelative Sizes in Histology

Standard Units of MeasureStandard Units of Measure

micrometer: 10-6 meter

nanometer: 10-9 meter

Relative Sizes in HistologyRelative Sizes in Histology

Standard Units of MeasureStandard Units of Measure

Changes in apparent shape, Changes in apparent shape, due to orientation of sectionsdue to orientation of sections

Ross, 5th ed, fig 1.11

Changes in apparent shape, Changes in apparent shape, due to orientation of sectionsdue to orientation of sections

Netter/Ciba

Junqueira fig 1-30

Epithelium

•No blood vessles, nourishment is by diffusion•Epithelium is innervated•polarization

Trachea

Epithelium

Classification of Epithelia•Number of cells1. Simple

2. Pseudostratified

3. Stratified

•Shape of surface cells1. Squamous

2. Cuboidal

3. Columnar

4. Transitional

Stomach (NO 25): mesothelium HE ×400

Simple squamous epi.

Small intestine (NO 1): endothelium HE × 400

Thyroid (NO 35): HE × 100

Thyroid: HE × 400

Simple cuboidal epi.

Small intestine (NO 1): villus HE × 400 (Drawing )

Simple columnar epi., Goblet cells, striated border (microvilli)

Trachea Pseudostratified ciliated columnar epi

Trachea: HE × 400

Pseudostratified ciliated columnar epi.

Esophagus (No.2)

Stratified squamous epi.

Esophagus: HE × 100

Transitional epitheliumTransitional epithelium

distended contractedBladderBladder

Bladder (NO33): HE × 40

Bladder: HE × 100 Transitional epi.

Bladder: HE × 400

Transitional epi.

The end!