Functional Genomics Research Stream qPCR Data Lab...

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Functional Genomics Research StreamResearch Meeting: November 30 2010

Closing Ceremonies

Final Reports

• Post on Results Central - Important

• When: Sunday, December 5 @ 10PM

• Professionalism, quality ...

• General introduction ...

• Conclusions, reflections ...

• Clear section titles (bold) ...

• Name, Date, Research Stream ...

qPCR Data

• New data is available ...

• Expect it to be fully analyzed in report ...

• Check Results Central

Lab Cleaning

• Post on Results Central

• Freezer Boxes ...

• Tubes in Racks in -20C ...

• Plates in 4C ...

• Incubator ...

• Pipette Calibration ...

• General lab cleaning ...

Internships, NSC

• Post(s) on Results Central

• Remember to register ...

• Consider your options moving forward ...

Evaluations

• eCIS email should have been sent ...

• Please participate ...

Take Home Messages

The Details Matter

• Independent of your long-term plans

• Reagents, pH, buffers ...

• Pipette technique ...

• Aseptic technique ...

• Sensitive reactions ...

• How a protocol or kit works ...

Consistency Matters

• Scientific procedures are a careful dance ...

• Timing, conditions, verified reagents matter ...

• Diagnostics are your friend ...

• Assumptions will bite you in the ...

• Control as much as possible ...

• Trust few peers ...

The Process Matters

• Scientific procedures are a careful dance ...

• The dance is wicked ugly at first ...

• Make a mess.

• Evaluate.

• Make less of a mess.

• Repeat.

Communication Matters

• Laboratory Issues: Reagents, concentrations, protocols, procedures.

• Scientific Issues: Papers, techniques, ideas.

• Thinking and communicating on your feet.

• The Elevator Pitch - Be Prepared

• Be sensitive to who your audience is ...

• Could you convince your mother?

Things Change

• Our basic understanding changes.

• Questions change.

• Social interest changes.

• Technology changes.

• People come, people go.

• Be prepared for change, predict it.

Life Comes in Seasons• Current Season: Perspective & Tool-belt

• Understand as much as possible; commitment not needed.

• Future Seasons:Vector, Focus, Achieve & Expertise

• You are whomever you want to be.

• Appreciate seasons for what they allow and bring no matter how limiting they may feel.

Leadership Matters

• Positions rarely given ...

• Opportunities must be sensed ...

• Reward often delayed ...

• Personality dependent ...

People Matter

• Remember when I said “Trust Few”?That was peers.

• Your future managers, advisors, bosses, etc are a major key to your growth, development and success.

• Select them carefully.

• People first, science second.

Your Life Matters

• Try to keep track of what you enjoy, what you do not.

• Make choices to enrich for the former.

• Don’t be passive.

• Make sacrifices, be patient.

• Be sensitives to seasons where choice is wide open to you. Don’t pretend it’s not.

• Enjoy other things. Do other things.

Questions?