Transcript of From Isolates to Genomes Tucson High School Biotechnology Course Spring 2010.
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- From Isolates to Genomes Tucson High School Biotechnology
Course Spring 2010
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- Jennifer Brum Originally from Las Vegas BS in Marine Science
(minor in Chemistry) MS in Oceanography PhD in Oceanography
Currently a post-doc in Dr. Matthew Sullivans lab at the University
of Arizona Mostly study aquatic viral ecology, but some
biogeochemistry Have conducted research in the North Pacific Ocean,
Hawaii coastal regions, Antarctica, Mono Lake (Sierras)
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- Every living organism has viruses that infect it we just have
to find them 1 microliter (l) 1 square mm (mm 2 ) 1,000 bacteria
10,000 viruses 1 mouthful: 500 million viruses 50 million bacteria
Viruses are host specific
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- Our Project: Isolate and evaluate viruses that infect 3 marine
cyanobacterial strains Prochlorococcus sp. Strain : MED4 Isolated
from: Mediterranean Sea Adapted to high light Prochlorococcus sp.
Strain : MIT9313 Isolated from: Gulf Stream Adapted to low light
Synechococcus sp. Strain : WH7803 Isolated from: Sargasso Sea
X
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- Virus Samples from Tara Oceans
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- Spain Africa Italy Mediterranean Sea
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- Virus Samples from Tara Oceans
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- The Process 1) Isolate viruses from Tara Oceans samples that
infect our 2 cyanobacteria cultures 2) Use PCR and DNA sequencing
to examine DNA sequences of specific genes in the isolated viruses
- Hypotheses! 3) Look at isolated viruses with TEM 4) Analysis of
sequenced viral genomes from last years class
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- Today: Start the Isolation Process Agarose Overlays molten
agarose (29 C) cyanobacteria culture virus sample Plate (side
view)
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- Isolation No Infection Infection !! plaques
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- The Plan 1/2 students Pro MED4 x 4 Tara Samples 1/2 students
Syn WH7803 x 4 Tara Samples 33 students Each Tara sample gets
screened 3 times on each cyanobacteria strain Replication!
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- Controls Positive ControlNegative Control cyanobacteria + known
virus cyanobacteria + no viruses
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- A bit about sterile technique There are bacteria in the air
There are bacteria on your hands There are bacteria in your hair
There are bacteria everywhere!