FlowJo 中文实用手册

I FlowJo support@flowjochina.com 400-680-5527

FlowJo FlowJo7.6.5 2012/06/20

FlowJo ......................................................................................................................................1 FlowJo ...............................................................................................................1 FlowJo(Windows) .........................................................................................................1 FlowJo ...............................................................................................................................5

FlowJo ......................................................................................................................6 ...........................................................................................6 .........................................................................................................................10

FlowJo ............................................................................................................13 .................................................................................................13 .....................................................................................................................16 .........................................................................................................................16

FlowJo ....................................................................................................................18 FlowJo..............................................................................18 FlowJo .........................................................................................................18 .....................................................................................................................................24

FlowJo ....................................................................................................................27 .........................................................................................................................27 .........................................................................................................................32 .........................................................................................................................35

FlowJo ............................................................................................................43 .................................................................................................................................43 FlowJo .........................................................................................................44 .........................................................................................................................45

PatrickUnderline

PatrickUnderline

PatrickUnderline

PatrickUnderline

II FlowJo support@flowjochina.com 400-680-5527

FlowJo ....................................................................................................47 .........................................................................................................................47 FlowJo .................................................................................................48 .................................................................................................51 .........................................................................................................................53

PatrickUnderline

III FlowJo support@flowjochina.com 400-680-5527

1 FlowJo support@flowjochina.com 400-680-5527

FlowJo

FlowJoLeonard HerzenbergFACS

90FlowJo

FlowJo

FlowJo

1. PC

Windows XPVistaWindows7

512MB

2.

OSX10.3

512MB

FlowJo(Windows)

http://www.flowjochina.com/content/download


V7.6.5

http://www.flowjochina.com/sites/default/files/7.6.5_X32.exe

FlowJo

FlowJo

NEXT


NEXT

NEXT

Install

Done


a. DongleFlowJo

b. Serial NumberUse

Serial Number

c. Get Trial LicenseFlowJo

Use Serial Number


d. DemoFlowJo

Run in Demo ModeDemoFlowJoDemo

FlowJo

FlowJo


FlowJo

,flowjochina

www.flowjochina.com/sites/default/files/demodata.rar

3 color compCy5PE comp.fcs

1. FlowJo

2. 3 color compFlowJo


3 color comp3 color

comp

Cy5PE comp.fcs


1) XSSC

SSC

2) YFSC

FSC

3)

:


1) XCy5PE:CD4YHistogram

2)

a.

CD4CD4


b.

CD4CD

a.

WORDPPT

b.

SVG

PNGEMFJPG


c. FlowJo

d.


e.

data001.JPGJPG, PNG, GIF, EMF,

SVG, PDF

FlowJoPNG

f.


FlowJo

FlowJo

FlowJo3-color-experiment931115-

B02-Sample01.FCS

1. 3-color-experimentFlowJo

2. 931115-B02-Sample01.FCS

XFSCYSSC


3. XFluor::CD3YFSC

CD3T


4. TXCy5PE::CD4Y

PhyEry::CD8

Q1CD8CD8+ T

Q2

Q3CD4CD4+ T

Q4


TREGCD4/CD25/FOXP3SSC vs. CD4

CD4+CD25 vs. FOXP3

CD25+/FOXP3+CD4+/CD25+/FOXP3+

TREG


FlowJo

FlowJo

FlowJo

1.

2.

3.

FlowJo

FlowJoFlowJo

4

http://www.video.flowjochina.com/v75%20Compensation_conv.html

1. FlowJo

3 color compCy5PE comp.fcs, FITC

comp.fcs, PE comp.fcs3-COLOR.FCS

Cy5PE, FITC, PE

PatrickHighlight

PatrickHighlight

PatrickHighlight


2.

Sample

FlourFITC comp.fcs


3. 23FlowJo

4.

>


(poor control )FlowJo

1. 1,2,3

FlowJoPE


1) PEcw_Size

2) Clear

2.

1) PEFSC/SSC


2) XPhyEry::aCD8Y

Histogram PEPE

a.

b.

3) PE-PE

PE+


3. FlowJo

3-color comp

>

0Log

0

LogicalFlowJo

3-color comp3-

COLOR.FCS

1. 3-COLOR

XCD3YCD8

gate135.1%


2. XYT

3. T

Change transform values


X-10-30

a.

b. FCS3.0FlowJoFCS2.0

FlowJo


FlowJo

Titration DataCD8Sample 1

Sample 2 ~ Sample 82

1.

Sample 1


XFL1-HYHistogram Sample 1

FL1-H+

RenameCD8+FL1-H-

CD8-

2.


CD8+

MedianParameter

FL1-HCD8FL1-H


(FL1-H):MedianCD8-CD8

FL1-H

3.

Sample 1Sample 2

Sample 2Sample

1


Sample 1

Titration Data

Sample 1

ALTSample 1

Titration Data

Sample 1


1.

Sample 1CD8+CD8-

(FL1-H):Median


2.

Keyword

Antibody Concentration

OK

3.

CD8+ (FL1-H) Median CD8- (FL1-H) MedianCD8

CD8MFI

OK


4.

MFI

1MFI1.

1.

Sample 1


FlowJoTitration Data

FlowJo


2. (Overlay)

1

Titration Data

(1) Sample 1Sample 2

Sample 1

(2) SpecifyControl

Sample 1Sample 1OK


(3)


2

Titration Data

(1) Sample 1CTRL

Sample 1

Sample 1


(2)


(3)

(4) coloringfilled

(5)


FlowJo

Annexin V-FITC/PI

Annexin V-FITC/PI

PSVAnnexin V-FITC

PS

PSPI

PS

AnnexinPI

Annexin V-FITC/PI

AnnexinAnnexin, PI


FlowJo

apoptosisZL-2011-12-20_400.fcs

1. FlowJo

XFSC-A,YSSC-A

2. SSC/FSC


XFITC,AnnexinV-FITCYPE-Texas,PI

1.

2.

Q1(AnnexinV-FITC)-/PI+

Q2: (AnnexinV+FITC)+/PI+

Q3(AnnexinV-FITC)+/PI-

Q4(AnnexinV-FITC)-/PI-


Q2+Q3

AnnexinV


FlowJo

G1

SG2MG1S

DNAG2DNA

M

DNAPIPI

DNARNA

DNA

DNA


FlowJo

cell cycle 5206.001PI

1.

5206.001XFSC-HY

SSC-H


2.

XFL2-W(width)YFL2-A(area)

BDFL2-A

FL2-WFL2-AFL2H

BeckmanXFL3-LIN,PI

YFL3-PEAK,PI


3. FlowJo

G1SG2G1CV

G2CV


http://www.flowjochina.com/content/tutorial-video#cellcycle

1.

RMS

RMS(root mean square error)

RMS3.85

2.

WatsonDean-Jett-Fox

RMSRMS

Could not fit.

Watson

Dean-Jett-Fox


3.

G1G2meanCV

1 G2G12

G1G2G1

2(2G1)G2G1G20.5

2 G1G2

G1mean

G1mean

3 CV

DNAG1G2CV

G1 CV

=G2 CVG2 CV G1 CV

CVCV

CV


1. RMSroot mean square errorRMS

2. G1G1

SS

G2G2

3. G1G1

G2G2

4. CVcoefficient of variationG0/G1

CVCV

G1 cvG1G1

G2 cvG2

Flowjo

FlowJo FlJ FlowJo

FlowJo 中文实用手册

Documents

Transcript of FlowJo 中文实用手册

北京市中小学生开放实践活动系统 科学实践活动 操作手册kfsj.bjedu.cn/Uploads/Ueditor/file/20171022/59ec66500a749.pdf · 科学实践活动 _操作手册 （机构管理员、机构老师）

实验室 生物安全手册 - World Health Organizationapps.who.int/medicinedocs/documents/s16535zh/s16535zh.pdf实验室 生物安全手册 第三版 世界卫生组织 日内瓦

Flowjo Guide V1 - flowcytometry-embl.deflowcytometry-embl.de/wp-content/uploads/2016/09/Flowjo-Guide_V1.… · We encourage our users to take the time and go through the tutorials,

TwinCAT 3 Function 实验指导手册tr.beckhoff.com.cn/pluginfile.php/23730/mod_resource... · 2019-04-19 · TwinCAT 3 Function 实验指导手册 ModbusTCP & TCP/IP Version 1.0

招聘实用手册(Doc 29)

Dir 605 A1 中文实用手册

《服装裁剪实用手册 上装篇》鲍卫军 陈荣富

义务教育课程标准实验教科书 数学一年级上册

Flowjo - Paris Descartes · FlowJo is an integrated environment for viewing and analyzing flow cytometric data, presented in the form of a Workspace. ... (Mac or PC) at the top of

人教实验版教材 四年级上册第五课

义务教育课程标准实验教科书 数学（三年级上册）

义务教育课程标准实验教科书 一年级下册

FlowJo Introduction Tutorial

义务教育课程标准实验教科书 二年级 下册

实实实时时时猫猫猫 产产产品品品和和和技技技术术术手手手册册册 · 务模式，解决独特需求。 本文档将重点提供WebRTC视频通信云产品的技术资料和开发指导。

本科生毕业实习考核手册 - njucm.edu.cnyxy.njucm.edu.cn/_upload/article/files/82/d8/1d578... · 毕业实习考核手册（学生用）相关表格填写须知 毕业实习考核

义务教育课程标准实验教科书数学 八年级下册

2016 香港首次公开招股实务手册

FlowJo Basic Tutorial - cmima.csic.es · FlowJo Basic Tutorial FlowJo software is used for the analysis of flow cytometry data. ... Instructions to guide you through the analysis

全日制聋校实验教材 第二册 语文

北京市中小学生开放实践活动系统科学实践活动操作手册kfsj.bjedu.cn/Uploads/Ueditor/file/20171022/59ec66500a749.pdf · 科学实践活动 _操作手册（机构管理员、机构老师）

实验室生物安全手册 - World Health Organizationapps.who.int/medicinedocs/documents/s16535zh/s16535zh.pdf实验室生物安全手册第三版世界卫生组织日内瓦

《服装裁剪实用手册上装篇》鲍卫军陈荣富

义务教育课程标准实验教科书数学一年级上册

人教实验版教材四年级上册第五课

义务教育课程标准实验教科书数学（三年级上册）

义务教育课程标准实验教科书一年级下册

义务教育课程标准实验教科书二年级下册

实实实时时时猫猫猫产产产品品品和和和技技技术术术手手手册册册 · 务模式，解决独特需求。本文档将重点提供WebRTC视频通信云产品的技术资料和开发指导。

本科生毕业实习考核手册 - njucm.edu.cnyxy.njucm.edu.cn/_upload/article/files/82/d8/1d578... · 毕业实习考核手册（学生用）相关表格填写须知毕业实习考核

义务教育课程标准实验教科书数学八年级下册

全日制聋校实验教材第二册语文