Christiane’s Super Crazy Complicated Difficult Challenging Super Crazy

ProfessionalGuide to DNA Extraction

Christiane’s Guide to DNA Extraction

1. Spit in a cup 2. Add a drop of soap 3. Add a pinch of salt and mix4. Add three spoonfuls of alcohol

Mouth

Saliva

Soap

DNA

DNA

Water

Phosphate Backbone (DNA)

Phosphate Backbone (DNA)

Salt

Alcohol

Christiane’s Guide to DNA Extraction

• Spit in a cup (~1mL of saliva)• Add a drop of soap and mix

You do not need a lot• Add a pinch of salt and mix• Add three spoonfuls of alcohol

– Cover the top of the container– Swirl the cup, but DO NOT mix the contents with a spoon,

otherwise you will break up the DNA into fragments– Allow for the contents to settle– If DNA is not visible after a few minutes, add another

spoonful of alcohol