Post on 04-Nov-2019
Laboratory of Cancer Biology and Cannabinoid Research
Technion
David (Dedi) Meiri
Cannabidiolic acid, a major cannabinoid in fiber-type cannabis, is an inhibitor of MDA-MB-231 breast cancer cell migration. Takeda S, Okajima S, Miyoshi H, Yoshida K, Okamoto Y, Okada T, Amamoto T, Watanabe K, Omiecinski CJ, Aramaki H.
Cannabidiolic acid-mediated selective down-regulation of c-fos in highly aggressive breast cancer MDA-MB-231 cells: possible involvement of its down-regulation in the abrogation of aggressiveness. Takeda S, Himeno T, Kakizoe K, Okazaki H, Okada T, Watanabe K, Aramaki H.
leukaemiaAcute lymphoblastic
(ALL) (AML) leukaemiaAcute myeloid
Anal cancer Bile duct cancer Bladder cancer
Bone cancer Bowel cancer
tumoursBrain Breast cancer
Cancer of unknown primary Cancer spread to bone Cancer spread to brain Cancer spread to liver Cancer spread to lung
Carcinoid Cervical cancer
Children's cancer Choriocarcinoma
leukaemiaChronic lymphocytic (CLL)
(CML) leukaemiaChronic myeloid Colon cancer
Colorectal cancer Endometrial cancer
Eye cancer Gallbladder cancer
Gastric cancer tumoursGestational trophoblastic
(GTT) leukaemiaHairy cell
Head and neck cancer
Hodgkin lymphoma Kidney cancer
Laryngeal cancer Leukaemia
Liver cancer Lung cancer Lymphoma
Melanoma skin cancer Men's cancer
Mesothelioma Molar pregnancy
Mouth and oropharyngeal cancer Myeloma
Nasal and sinus cancers Nasopharyngeal cancer
Non Hodgkin lymphoma (NHL) cancer Oesophageal
Ovarian cancer Pancreatic cancer
Penile cancer Prostate cancer
Rare cancers Rectal cancer
Salivary gland cancer Secondary cancers
Skin cancer (non melanoma)
Soft tissue sarcoma Stomach cancer
Testicular cancer Thyroid cancer
Unknown primary cancer Uterine cancer Vaginal cancer
cancer Vulval Womb cancer
Women's cancers
CannaCollage – Israel’s strains Dedi Meiri, Ph.D. Assistant Professor | Faculty of Biology Laboratory of Cancer Biology and Cannabinoid Research
Teva Adir
Seach
IMC Canndoc
Sheifa Lachaim Pharmocann
Better
Tikun Olam
DMSO Extract1 THC CBD
Extract2 CBD THC
Extract3 CBD THC
רפאל משולם
Different extract from CBD high cannabis plant
Cont CBD1 CBD2 CBD3 CBD4 CBD5 CBD6 CBD7
Tonic-clonic seizures
ImageXpress micro (IXM) analysis
The effect of pure Δ9THC on cancer cell survival
A375 melanoma cell line
THC µ g/ml %
D e
a d c
e l l s
2 4 8
0
20
40
60
80
100 A375
A375 melanoma cell line
U-87 cell vability48 Hours from treatment
CNB1CNB2
CNB3CNB4
CNB6CNB8
0
25
50
75
100
125
4 ug/ml
Cannabis Extract
cell
viab
ility
(% o
f con
trol
)
PC3- cell viability48 Hours from treatment
CNB1CNB2
CNB3CNB4
CNB6CNB8
0
25
50
75
100
125
4ug/ml
cannabis extract
cell
viab
ility
(% o
f con
trol
)
1 2 3 4 5 6
glioblastoma Prostate cancer
1 2 3 4 5 6
Conversion of THCA to THC
O
COOH
OH
CH3
CH3
CH3
CH3 O
OH
CH3
CH3
CH3
CH3
to or h
- CO2
Tetrahydrocannabinolic acid, "inactive" (THCA)
C22H30O4, MW = 358.47
transTetrahydrocannabinol, "active" (THC)
C21H30O2, MW = 314.46
O
OH
CH3
CH3
CH3
CH3
Cannabinol,"slightly active" (CBN),
C21H26O2, MW = 310.43
- 4H
41
U-87 cell vability48 Hours from treatment
CNB1CNB2
CNB3CNB4
CNB6CNB8
0
25
50
75
100
125
4 ug/ml
Cannabis Extract
cell
viab
ility
(% o
f con
trol
)
PC3- cell viability48 Hours from treatment
CNB1CNB2
CNB3CNB4
CNB6CNB8
0
25
50
75
100
125
4ug/ml
cannabis extract
cell
viab
ility
(% o
f con
trol
)
1 2 3 4 5 6
glioblastoma Prostate cancer
1 2 3 4 5 6
No mutation Mutation 50% activity Mutation 30% activitv Mutation no activity
DATA BASE PROJECT
Optimize personalized Cannabis treatment
Cannabis strain database
Mouse models
Clinical Trails
Patient database Cannabis growers: Personalized strain
usage history
Foreigner clinics
Smart Phone - App4Cannabis:
Report symptoms and view optimized treatments
Aim 2
Aim 1
Mass-spec based metabolomics profiling
Computational analysis via machine learning
Aim 3 Lab research on therapeutic potential
of specific Cannabis strains
Clinical trials
Maximizing therapeutic efficacy while minimizing side effects
Aim 4
Optimize personalized Cannabis treatment
Cannabis strain database
Mouse models
Clinical Trails
Patient database Cannabis growers: Personalized strain
usage history
Foreigner clinics
Smart Phone - App4Cannabis:
Report symptoms and view optimized treatments
Aim 2
Aim 1
Mass-spec based metabolomics profiling
Computational analysis via machine learning
Aim 3 Lab research on therapeutic potential
of specific Cannabis strains
Clinical trials
Maximizing therapeutic efficacy while minimizing side effects
Aim 4
Reducing anxiety and violence in autistic kids
Reducing anxiety and violence in autistic kids
Reducing anxiety and violence in autistic kids
Optimize personalized Cannabis treatment
Cannabis strain database
Mouse models
Clinical Trails
Patient database Cannabis growers: Personalized strain
usage history
Foreigner clinics
Smart Phone - App4Cannabis:
Report symptoms and view optimized treatments
Aim 2
Aim 1
Mass-spec based metabolomics profiling
Computational analysis via machine learning
Aim 3 Lab research on therapeutic potential
of specific Cannabis strains
Clinical trials
Maximizing therapeutic efficacy while minimizing side effects
Aim 4
CK Sience
MEIRI’S LAB