CS-CA-GFP map - Riken...CS-CA-MCS 9679 bp MluI 228 NotI 1521 PstI 2573 EcoRI 2579 ApaI 3424 XbaI...

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Transcript of CS-CA-GFP map - Riken...CS-CA-MCS 9679 bp MluI 228 NotI 1521 PstI 2573 EcoRI 2579 ApaI 3424 XbaI...

CS-CA-MCS

9679 bp

MluI 228

NotI 1521

PstI 2573EcoRI 2579

ApaI 3424

XbaI 4188

(PstI) 4311(EcoRI) 4317(PstI) 4322BstXI 4332(NotI) 4343XhoI 4350

ApaI 5661

CMV RU5

5' SD

RRE

3' SAcPPT+CTS

CAG

MCSPREdel U3RU5

BGH pAf1 ori

SV40 pro & ori

Zeo

SV40 pA

ColE1 ori

Amp 1

Miyoshi
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Plasmid name: CS-CA-MCS Plasmid size: 9679 bp Note CMV: Human cytomegalovirus immediate early promoter y: Packaging signal 5’SD: 5’ splicing donor site 3’SA: 3’ splicing acceptor site RRE: Rev responsive element cPPT: Central polypurine tract CTS: Central termination sequence CAG: CMV immediate early enhancer and chicken β-actin gene promoter MCS: Multiple cloning site PRE: Woodchuck hepatitis virus posttranscriptional regulatory element del U3: Deletion of enhancer and promoter sequences in the U3 region BGH pA: Bovine growth hormone polyadenylation signal SV40 pro & ori: SV40 early promoter and origin Zeo: Zeocin resistance gene SV40 pA: SV40 polyadenylation signal Amp: Ampicillin resistance gene
Miyoshi
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y