Climate Change, Disease, & Immunity Climate Change, Disease, & Immunity in Coralin Coral

Kevin B. Strychar1 & Paul W. Sammarco2

1Texas A&M University – Corpus Christi2Louisiana Universities Marine Consortium

Introduction

Introduction

Bruno, 2010Cook, 2011

Introduction

Introduction

Harvell et al., 2007Caribbean

JM Cervino, pers. comm.

JM Cervino, pers. comm.

Many different bacterial populations associated with various parts of coral (Frias-Lopez et al., 2002)

Populations change with different environmental conditions (Koren and Rosenberg, 2006)

Changes hypothesized to be resistance mechanism for coral (Reshef et al., 2006)

Introduction

Kerr et al., 1972Gates et al., 1999

Strychar et al., 2001Strychar et al., 2004

Bleaching as a pathogenic response…

Introduction

Types of Programmed Cell Death (PCD)

= PCD

Apoptosis

Autophagic

Caspase-independent

Anolkis

Aponecrosis

Apoptosis – Aponecrosis - Necrosis

programmed traumatic

Who does the lions share?

Methods

Methods

Annexin V

PI

34C

Apoptotic

Results

3 6 9 12 3 6 9 12Time (h)

Pe

rce

nt

(%)

of

ce

lls

in

-sit

u

Viable Cells

ResultsNecrotic cells

Pe

rce

nt

(%)

of

ce

lls

in

-sit

u

3 6 9 12Time (h)

3 6 9 12

ResultsApoptotic Cells

3 6 9 12

Pe

rce

nt

(%)

of

ce

lls

in

-sit

u

3 6 9 12Time (h)

Results

28C 30C

B

32C

Normal mid-apoptoticC

32C

Normal mid-necrotic D

34C

Apoptotic

34C

Necrotic

Apoptosis of Symbiodinium from host Acropora hyacinthus

E F

A

Results

Apoptosis of host Acropora hyacinthus

Summary

“Trogocytosis”: The signaling molecules released by one cellact on neighboring target cells (plasma membrane-like

transmitters).- e.g. CDKs – cell dependent kinases

Next step: in progress