Chromatography研習資料

Separation of Pigments

In a solvent according

To their densities

Separate plant pigments

Also for Forensics

• Find type of ink

(different color separations)

• Find impurities in products

Taper Strip

• Cut to a point with scissors

Mark a starting line

• Use pencil

• (not ink)

Chemical Application

Add small drops, let dry, add again until dark spot

KEEP ABOVE PENCIL LINE

Use coin (mechanical) – geranium leaf

• Use serrated coin

• Do a few times to make a dark line

Be sure liquid is below line

• Attach paper to paper clip

• Attach paper clip to stopper

Chromatography Animation

Paper Chromatography Videos

Chromatography Over Time

• Time Lapse Video• (Be patient and give it

time to move)

Mark bands of colorsLighter pigments will move farther up the paper

Heavier pigments will remain near the start

Pencil line will mark start point

Separation of Ink

Calculating Rf

• Compares distance the solvent traveled to the distance the test sample traveled.

• Rf = distance of test sample

• distance solvent

Measure tops of colors

• From starting point at bottom to top of each color

Distance

of solvent

Pigments in Plants

• carotene-yellow orange

• chlorophyll a-bright green

• Xanthophylls-yellow

• chlorophyll b-khaki green

More colors

• phycocyanin = bluish• phycoerythrin = reddish

Carrier Solution

• Stored in da hood!

• Return the waste to the waste container in da hood.

• Boy-e-e-e-e-e-e-e-e-e-e!

Sample Results

Click Here for Examples

• Results of Chromatograph

Accessory Pigments

• fucoxanthin = brown

• Phycobilin = reds

• phycocyanin = bluish

Conclusion Question #2