Post on 19-Dec-2015
Case Study: Controlling exposure to isoflurane used in animal surgical procedures in Research & Development
Michel Vangeel, Janssen Campus Belgium
IOHA London 2015, 25-30 April 2015 Hilton London Metropole
Introduction
• Janssen Research & Development• All stages of Discovery • 1800 employees• Beerse, Belgium• Johnson & Johnson
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
Anticipation
• Reports acute adverse health effects• During animal surgical procedures • Headaches, dizziness, loss of concentration,
numbness fingers;• 3 Janssen R&D sites J&J• Isoflurane exposures 10-23 ppm range• Internal J&J OEL: 20 ppm
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
IdentificationBasic characterization: isoflurane
• Boiling point: 48.5 °C (1013 mbar) - Vapor pressure: 440 hPa (25°C) - Relative Density: 1.45 g/cm3
• Routes of entry: Inhalation - may provoke:Headache, dizziness, loss of concentration, unconsciousness
• In case of contact with eyes: irritation, redness
• Occupational Exposure Limits (IFA - GESTIS databank)
- Canada: 2 ppm- Sweden, Finland, Austria: 10 ppm – 20 ppm (STEL)- J&J: 20 2 ppm
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
IdentificationBasic characterization: work stations & activities
Isoflurane evaporator Surgical interventions (Masks)
Induction Box
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
Filled with mixture of air and 2-5 volume % isoflurane minimum 30% O2 (till 100%), flow rate 1l/min
EvaluationExposure monitoring
• Identification work stations & sampling plan
• Personal & area monitoring:- SKC Anasorb 747 (100/50 mg)- flow rate: 200 cc/min.
• Continuous monitoring:- Thermo SaphIRe MIRAN- detection limit 0.04 ppm
• Statistical Analysis results- IDHA – Bayesian stats UCL95,75
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
ControlInduction box
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
= creates under pressure
• Controlled supply• Adjusted diameters &
position supply & exhaust• Glide lid in 2 steps• Adjusted sizes/animal• Speed removing• Additional exhaust
ControlAnesthetic masks
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
• Supply isoflurane inside• Exhaust outside• Avoid to cover whole table• Controlled supply isolfurane
with indication on screen
ControlWorkstations: correct use LEV
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
• Correct use of supply & exhaust- No disturbances flow pattern = recipients & equipment / covers (sheets)- Close valve supply on time- Proper connections tubing
• Isoflurane filters- Saturated filters = source of exposure- Good maintenance & follow up- Evaluate removal & connection with LEV
• Expansion LEV- No improvisation/bad connections
ControlWork practices & training
IOHA London 2015, 27-30 April 2015, Hilton London Metropole
• Wipe of fur- Wipe off fur animal in front of extraction unit- Reduction of 10 times the concentration- Without wiping: 60- 88 ppm- With (5 times) : 1.7 – 6.8 ppm
• Manipulations- Opening lid induction box: 2 steps- Speed removal animals
Conclusion
• Connection & teamwork OH, IH, toxicology, EHS colleagues, business
• Respect pillars of Industrial Hygiene• Control: combination of engineering controls,
good work practices and training & awareness
• Reduce exposure < 2 ppm (new J&J OEL)
IOHA London 2015, 27-30 April 2015, Hilton London Metropole