Post on 09-Mar-2021
ANALYTICALSOLUTIONSA U S T R A L I A
Cannabinoid Analytical and Preparative Separations
Supercritical Fluid Chromatography (SFC) is a relatively new technique that offers several advantages including faster run times, better resolution and more concentrated separations.The limitations of SFC are: it has only been proven on small molecular groups and gradient development is more complicated than for HPLC. The cannabinoid group is one application that works well on SFC. The main cannabinoid species that require separation are Cannabidivarin, Cannabidiol, Cannabichromene, Tetrahydrocannabivarin, Tetrahydrocannabinol,Cannabinol, Cannabigerol, Cannabidiolic acid, Tetrahydrocannabinolic acid and Cannabigerolic acid.
Column type:
Eluent:
Flow rate:
Oven °C:
Back P:
Detection:
Phenomenex Lux, 5µm
CO and co-solvent Ethanol
5ml/min
24
100 bar
DAD @ 220nm
2
ANALYTICAL SEPARATION OF CANNABINOIDS BY SFC
Separation conditions: Cannabinoids Chemical Structures
1. Cannabidivarin (CBDV)2. Cannabidiol (CBD)3. Cannabichromene (CBC)4. Tetrahydrocannabivarin (THCV)5. Tetrahydrocannabinol (THC)6. Cannabinol (CBN)7. Cannabigerol (CBG)8. Cannadiolic Acid (CBDA)9. Tetrahydrocannabinolic Acid (THCA)10. Cannabigerolic Acid (CBGA)
6
Time (min)
Nor
mal
ised
resp
onse
(220
nm)
45
3
21
7 89
10
1. Cannabidivarin (CBDV)
3. Cannabichromene (CBC)
5. Tetrahydrocannabinol (THC)
7. Cannabigerol (CBG)
9. Tetrahydrocannabinolic acid (THCA)
2. Cannabidiol (CBD)
4. Tetrahydrocannabivarin (THCV)
6. Cannabinol (CBN)
8. Cannabidiolic acid (CBDA)
10. Cannabigerolic acid (CBGA)