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Bioinforma)csResources-Swissprot-

Lecture&ExercisesProf.B.Rost,Dr.L.Richter,J.Reeb

Ins)tutfürInforma)kI12

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Puta)veSchedule

Apr. 22nd Intro, General Overview (1. sh.) Jun 10th No-SQL (7.sh.) Apr. 29th Sequence Databases (2. sh.) Jun 17th No-SQL (8.sh.)* May 6th No lecture Jun 24th JavaScript / UI (9.sh.) May 13th Sequence Databases (3. sh.) Jul 1st Web Services (10.sh.) May 20th Structure Databases (4. sh)* Jul 8th Bioinformatics Suites / Forums May 27th SQL (5. sh.) Jul 15th Wrap Up, Q&A Jun 3rd SQL (6. sh) Jul 28th Exam, 10:30-12:00 MW1050

* These exercises can earn you a bonus

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XMLInfusion(in10sec)●  compila)onfromhMp://www.w3schools.com/xml/default.asp

●  XMLisasoQware-andhardware-independenttooltostoreandtotransportdata

●  XMLstandsforeXtensibleMarkupLanguage

●  designedtostoreandtransportdata●  designedtobeself-descrip)ve

●  W3Crecommenda)on

●  itdoesNOTDOanything

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AboutTags

●  XMLtagsarenotpredefinedlikeHTMLtags●  everybodycan/hastoinventhisowntags

●  newtagscanbeaddedany)me

●  theauthorhastodefinecontentandstructureofthedocument

●  everythingisplaintext

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DocumentStructure<?xml version="1.0" encoding="UTF-8"?>!<bookstore>!!  <book category="cooking">!    <title lang="en">Everyday Italian</title>!    <author>Giada De Laurentiis</author>!    <year>2005</year>!    <price>30.00</price>!  </book>!! <book category="children">!    <title lang="en">Harry Potter</title>!    <author>J K. Rowling</author>!    <year>2005</year>!    <price>29.99</price>!  </book>!!....!</bookstore>!!takenfromhMp://www.w3schools.com/xml/xml_usedfor.asp

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SyntaxRules●  elementsaredefinedusingtags:<tagName> ... </tagName>or<tagName/>!

●  elementscanbenested(containotherelements-parentandchildnodes,siblingnodes)

●  elementscanhavetextcontent

●  eachdocumentmustcontainONErootelementthatistheparentofallotherelements

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SyntaxRefined

●  prologline<?xml ...>isop)onal●  tagsmustbe(self-)closed

●  tagarecasesensi)ve

●  tagsmustbeproperlynested:<a><b>....</a></b> Wrong!<a><b>....</b></a>! Right!

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SyntaxRefined●  tagsmayhaveaMributes●  aMributevaluesmustalwaysbequoted

●  somespecialcharacterscannotbeuseddirectly

●  ->codedbyen)tyreferences:&lt; < lessthan&gt; > greaterthan&amp; & ampersand&apos; ‘ apostrophe&quot; “ quota)onmark

●  comments:<!-- .... -->!

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TagNames●  casesensi)ve●  muststartwithaleMerorunderscore

●  mustnotstartwiththeleMersxmlinanycase

●  cancontain:leMers,digits,hyphens,underscoresandperiods

●  cannotcontainspaces

●  applycommonsenseandaconsistentstyle●  avoid:minus(-),period(.),colon(:),non-englishcharactersforcompa)bilityreasons

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XMLElement

●  everythingbetweenthestartandtheendtag●  tagsareincluded

●  cancontain:-  text-  aMributes-  otherelements-  amixofall

●  areextensible

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XMLAMributes

●  valuesmustbequoted:singleordoublequotes●  theunusedcharactercanbeusedinsidethevalue

●  decisionforaMributeorelementundecided,but:-  aMributescannotcontainmul)plevalues-  aMributescannotcontaintreestructures-  aMributesarenoteasilyexpandable

●  usefultostoremetadata,likeelementid,etc.

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AGlimpseofNamespaces

●  allowtopreventtagnamecollisionsbetweendifferentauthors/applica)ons/domains

●  implementedbytheintroduc)onofprefixes●  definedasanaMribute:xmlns:prefix=“URI”!

●  usage:<prefix:tagName>!●  theURIisonlyneededtobeunique

●  usedtointegrateotherspecifica)ons,e.g.XSLT

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LevelsofCorrectness●  wellformed:adocumentobeythesyntaxrules:-  rootelement-  closingtag-  casesensi)ve-  properlynested-  aMributevaluesquoted

●  validdocuments:inadd)ontobeingvalidthealsoconformtoadocumenttypedefini)on(formatspecifica)on)

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DocumentTypeDefini)ons

●  twowaystospecifyadocumentstructure:●  DTD:DocumentTypDefini)on

●  XMLSchema:XMLbasedalterna)vetoDTD

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Example

<?xml version="1.0" encoding="UTF-8"?> <!DOCTYPE note SYSTEM "Note.dtd”> <note> <to>Tove</to> <from>Jani</from> <heading>Reminder</heading> <body>Don't forget me this weekend! &copyright; </body> </note>!

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Example

<!DOCTYPE note [ <!ELEMENT note (to,from,heading,body)> <!ELEMENT to (#PCDATA)> <!ELEMENT from (#PCDATA)> <!ELEMENT heading (#PCDATA)> <!ELEMENT body (#PCDATA)> <!ENTITY copyright “Copyright by ..”> ]>!

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XMLDTD

●  referencedfromadocumentwith:<!DOCTYPE note SYSTEM "Note.dtd">!

●  !DOCTYPEdefinestherootelement●  !ELEMENTdefinesthestructureoftheelements

●  #PCDTAmeansparse-abletextdata●  !ENTITYdefinesspecialcharactersorstrings

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XMLSchema●  alterna)vetoDTD<xs:element name="note”> <xs:complexType> <xs:sequence> <xs:element name="to" type="xs:string"/> <xs:element name="from" type="xs:string"/> <xs:element name="heading" type="xs:string"/> <xs:element name="body" type="xs:string"/> </xs:sequence> </xs:complexType> </xs:element>!

●  supportofdatatypesandnamespaces

●  wriMeninXMLandextensible!

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NamesandOtherComplica)ons

AmosBairoch

takenfromhMp://web.expasy.org/images/people/Amos_Bairoch.jpg

IoannisXenarios

takenfromhMp://www.isb-sib.ch/people/Ioannis.Xenarios

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History

1986 A.BairochcreatedSwiss-Protatthe UniversityofGeneva,since1988in

collabora)onwithEMBL/EBI

1993 togetherwithRonAppellaunchofExPASy

1998 Founda)onofSIB(SwissIns)tuteof Bioinforma)cs)

2002 Founda)onoftheUniProtconsor)umby EBI,SIBandPIR

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UniProtComponents:●  UniProtKB:-  UniProtKB/Swiss-Prot-  UniProtKB/TrEMBL

●  UniParc:puresequencearchive,noannota)ons

●  UniRef:consistsfothreedatabasesofclusteredsetsofproteinsequences(UniRef100,UniRef90,UniRef50)usingtheCD-HITalgorithm

●  UniMes:datafrommetagenomicandenvironmentalsamples,notinUniProtKB

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ExPASy

●  hMp://www.expasy.org●  ExpertProteinAnalysisSystem(1993)

●  now:SIBExPASyBioinforma)csResourcesPortal●  Ar)moP,JonnalageddaM,ArnoldK,Bara)nD,CsardiG,de

CastroE,DuvaudS,FlegelV,For)erA,GasteigerE,GrosdidierA,HernandezC,IoannidisV,KuznetsovD,Liech)R,MoreoS,MostaguirK,RedaschiN,RossierG,XenariosI,andStockingerH.ExPASy:SIBbioinforma9csresourceportal,NucleicAcidsRes,40(W1):W597-W603,2012.

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ExpasyCategories

●  Proteomics●  Genomics

●  StructuralBioinforma)cs

●  Systemsbiology●  Phylogeny/evolu)on

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ExpasyCategories

●  Popula)ongene)cs●  Transcriptomics

●  Biophysics

●  Imaging●  DrugDesign

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ResourceDescrip)on

1.  Resourcenameanddescrip)on2.  MaintainingSIBgroup

3.  Scien)ficcategory4.  Keywords:acontrolledvocabularyisusedtotag

theresource

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ResourceDescrip)on

5.  URLforthewebinterfaceandforthedownloadifavailable

6.  SoQwaretype:website,commandlineinterface,GUI,etc

7.  Status:greencheckboxifcurrentlyavailable

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UniProt/SwissProtSta)s)cs

●  Release2016_05,May.11th●  takenfromhMp://web.expasy.org/docs/relnotes/relstat.html

●  551.193sequenceentries(548.454in2015_05)/196.822.649aminoacids(195.409.447in2015_05)

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UniProt/SwissProtSta)s)cs●  Growthoveroneyear:2016_5vs2015_5

Protein existence (PE) Entries % 1. Evidence at protein level 92.536

(85.419) 16.8

(15.6) 2. Evidence at transcript level 57.757

(61.814) 10.5

(11.3) 3. Inferred from homology 387.589

(387.733) 70.3

(70.7) 4. Predicted 11358

(11.526) 2.1

(2.1) 5. Uncertain 1.953

(1.962) 0.4

(0.4)

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Development

takenfromhMp://web.expasy.org/docs/relnotes/relstat1.pngforrelease2015_5

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MoreNumbers(rel.2015_5)

●  Representedspecies:13.209●  Top20species:116.206sequences,i.e.21.3%ofthetotalnumberofsequences

Entries No of Species Entries No of Species 1 5.495 8 228 2 1.899 9 214 3 1.023 10 122 4 657 11-20 711 5 487 21-50 426 6 399 51-100 213 7 289 >100 1.046

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SpeciesRepresenta)on(rel.2015_5)Top Frequency Species

1 20.198 Homo sapiens (Human) 2 16.711 Mus musculus (Mouse) 3 13.888 Arabidopsis thaliana (Mouse-ear cress) 4 7.921 Rattus norvegicus (Rat) 5 6.718 Saccharomyces cerevisiae (Baker’s yest) 6 5.993 Bos taurus (Bovine) 7 5.103 Schizosaccheromyces pombe (Fission yeast) 8 4.433 Escherichia coli K12 9 4.185 Bacillus subtilis 10 4.131 Dictyostelium discoideum (Slime mold) ... ... ...

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Representa)onoftheDivisions(rel.2015_5)

Archaea (4%), 19340

Bacteria (61%), 332110

Eukaryota (33%), 180411

Viruses (3%), 16593

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Distribu)onofEukaryota(rel.2015_5)

Human (11%), 20199

Other Mammalia

(26%), 46146

Other Vertebrata

(10%), 17823

Viridiplantae (20%), 36480

Fungi (17%), 31527

Insecta (5%), 8781

Nematoda (2%), 4417

Other (8%), 15038

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LengthDistribu)on(rel.2015_5)

0

10000

20000

30000

40000

50000

60000

70000

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AminoAcidComposi)on(rel.2015_5)

figure taken from http://web.expasy.org/docs/relnotes/relstat.html gray=aliphatic, red=acidic, green=small hydroxy, blue=basic, black=aromatic, white=amide, yellow=sulfur

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SwissProtAnnota)onProcess

●  definedinhMp://www.uniprot.org/docs/sop_manual_cura)on.pdf

●  explainedinhMp://www.uniprot.org/help/manual_cura)on

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Annota)onPhases

1.  Sequencecura)on2.  Sequenceanalysis3.  Literaturecura)on4.  Family-basedcura)on5.  EvidenceaMribu)on6.  Qualityassurance,integra)onandupdate

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SequenceCura)on

●  morethan95%aretranslatedCDSfromINSDC●  othersources:PDB,directproteinsequencing,projectsnotsubmiongtoINSDC

●  sequencesareselectedaccordingtocura)onpriori)es(hMp://www.uniprot.org/program/)

●  resultsinthe“canonicalsequence”foragene/speciespair

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Stepstowardthecanonicalsequence

●  Entryselec)on●  RunBLASTsimilaritysearchestoiden)fyaddi)onalsequencesforthesamegene

●  Iden)fyhomologsbyreciprocalBLASTandphylogenybasedresources

●  Lockselectedentriesforothercuratorstopreventduplica)on

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Stepstowardthecanonicalsequence●  PreparesequencealignmentswithT-Coffee,Muscle,ClustalW

●  Mergeintothecanonicalsequence:-  mostprevalent-  mostsimilartoorthologssequencesfoundinotherspecies

-  basedonlengthandaacomposi)onitallowstheclearestdescrip)on

-  default:longest

●  recordconflictsandvaria)ons

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SequenceAnalysis

●  Severalanalysisprogramsareappliedtothesequencesfor:-  topologicalfeatures-  post-transla)onalmodifica)ons-  domains

●  allresultsaremanuallycheckedandin-orexcludedforannota)on

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TopologicalAnalysis

Tools Prediction Signal P Presence and location of signal peptides TargetP Presence and location of transit peptides Predotar Mitochondrial, plastid or ER targeting sequences ESKW Transmembrane domains MEMSAT Transmembrane domains TMHMM Transmembrane domains Phobius Discriminates transmembrane and signal regions

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Post-transla)onalmodifica)onAnalysis

Tools Prediction GPI-predictor GPI lipid anchor sites NetNGlyc N-glycosylation sites NetOGlyc O-glycosylation sites NMT Predictor N-terminal myristoylation sites Sulfinator Tyrosine sulfatation sites

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DomainAnalysis

Tools Prediction ps_scan internal PROSITE profile, pattern and rule scanning InterPro retrieves non-PROSITE motif matches using InterPro database or

InterProScan Coils Coiled-coils regions polyAA internal program which identifies homopolymeric stretches of amino

acids REPEAT identifies the following repeats: Ankyrin, Armadillo, HAT, HEAT,

Kelch, Leucine-rich, PFTA, PFTB, RCC1, TPR, WD40

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Automatically selected results are returned in a graphical interface which allows visualisation of the predictions (Figure 1). Selected features are shown in green and unselected features are shown in red. The selected/unselected state of a feature can be toggled by clicking on it.

Figure 1. UniProtKB sequence analysis results displayed in graphical interface

All predictions are manually reviewed and relevant results are selected for inclusion in the entry. The sequence analysis platform then transforms the selected features into UniProtKB annotation by applying a set of automatic annotation rules (Figure 2).

taken from http://www.uniprot.org/docs/sop_manual_curation.pdf

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LiteratureCura)on

●  Iden)fica)onofrelevantscien)ficliteraturefrom-  literatureandtextminingresources(PubMed,EuropePMC,iHOP,TextPresso)

-  addi)onsfromothersourcesmadebythecurator

●  Informa)onisextractedformthefulltext:-  generalannota)ons(notposi)onspecific)-  posi)onspecificannota)ons

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GeneralAnnota)ons

●  hMp://www.uniprot.org/help/general_annota)on

●  posi)on-independent●  containsmostlygeneralbiologicalinforma)onlike:func)ons,cataly)cac)vity,cofactor,enzymeregula)on,subunitstructure,pathway,...

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SequenceAnnota)ons

●  posi)ondependent●  hMp://www.uniprot.org/help/sequence_annota)on

●  regionsorsitesofinterestlikepost-transla)onalmodifica)ons,bindingsites,ac)vesites,etc.

●  containsseveralsubsec)ons:moleculeprocessing,regions,sites,aminoacidmodifica)ons,naturalvariants,experimentalinfo,secondarystructure

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Family-basedCura)on

●  Evalua)onandcura)onofhomologsasdescribedabove

●  Standardiza)onofannota)onofhomologs●  Propaga)onofannota)onacrossthehomologstoensureconsistency

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EvidenceAMribu)on●  Everyannota)onisaMributedtoitsoriginalsource

●  Everyannota)oncanbetracedbackandevaluated

●  Forevidencedis)nc)onthereare7codesfromtheEvidenceCodeOntology(ECO)usedformanuallycuratedentries

●  hMp://www.uniprot.org/help/evidences●  Addi)onalGOtermannota)on

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done through the use of a subset of evidence codes from the Evidence Code Ontology (ECO) (24). There are seven ECO evidence codes used in manually curated entries as shown in Table 2.

Table 2. Evidence Code Ontology (ECO) codes used during the UniProt manual curation process

ECO code Term name Usage ECO:0000269 experimental evidence used in

manual assertion Information for which there is published experimental evidence

ECO:0000303 non-traceable author statement used in manual assertion

Information based on author statements in scientific articles for which there is no experimental support

ECO:0000250 sequence similarity evidence used in manual assertion

Information which has been propagated from a related experimentally characterised protein

ECO:0000312 imported information used in manual assertion

Information which has been imported from another database and manually verified

ECO:0000305 curator inference used in manual assertion

Information which has been inferred by a curator based on his/her scientific knowledge or on the scientific content of an article

ECO:0000255 match to sequence model evidence used in manual assertion

Information originating from the UniProt automatic annotation systems or any of the sequence analysis programs used during the manual curation process and which has been manually verified

ECO:0000244 combinatorial evidence used in manual assertion

Information which is manually curated based on a combination of experimental and computational evidence

Full details of the evidences used in UniProtKB are available at http://www.uniprot.org/manual/evidences. 4.11 GO annotation Gene Ontology (GO) terms are assigned based on experimental data from the literature. Relevant terms are identified using the QuickGO (25) browser and are assigned to entries using the Protein2GO curation tool. This tool has been developed within the UniProt group and is used both by UniProt and by other members of the GO Consortium. GO terms are also propagated to homologous proteins where appropriate. The procedure is described in more detail at http://www.ebi.ac.uk/GOA/ManualAnnotationEfforts. 4.12 Quality control and integration All finished entries are run through a series of automated checks which verify a large number of biological rules such as the positions and relevance of amino acids cited in the entry. Any reported errors are corrected. Once an entry has passed the automated checks, it undergoes manual review by a senior curator to ensure that all relevant sequences have been merged, that all relevant literature has been added, that the annotation has been added correctly, and that all relevant sequence analysis results have been included. Once an entry has passed the automated and manual quality control checks, it is integrated into the database. 4.13 Unlock finished entries Integrated entries are unlocked so that they are available for further curation.

taken from http://www.uniprot.org/docs/sop_manual_curation.pdf

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QualityControlandIntegra)on

●  Finishedentriesrunthroughaseriesofrule-basedcheckedconcerningespeciallyposi)onsandregions

●  Allerrorsarecorrected

●  Manuallyreviewedbyaseniorcurator

●  Finallyitisintegratedintothedatabase●  Unlockthefinishedentriesforfurthercura)on

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Demostra)on

●  hMp://www.uniprot.org/uniprot/P62756#sec)on_features

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TheSwiss-ProtFlatFile●  hMp://web.expasy.org/docs/userman.html●  Anentryiscomposedbydifferentlinetypes

●  Linetypeshavetheirownformat

●  FollowsEMBLNucleo)deSequenceDatabaseformatascloseaspossible

●  2sec)ons:-  coredata(sequencedata,cita)oninfo,taxonomy)-  annota)ons(func)on,modifica)on,domains,secandquartstructure,diseaseassocia)ons,conflicts,asf)

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Line Code

Content Occurence in an entry

ID Identification Once; starts the entry AC Accession number(s) Once or more DT Date Three times DE Description Once or more GN Gene name(s) Optional OS Organism species Once or more OG Organelle Optional OC Organism classification Once or more OX Taxonomy cross-reference Once OH Organism host Optional

--continued--

The following table lists the available two-letter line codes. Each code is followed by three blanks.

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Line Code

Content Occurence in an entry

RN Reference number Once or more RP Reference position Once or more RC Reference comment(s) Optional RX Reference cross-reference(s) Optional RG Reference group Once or more (Optional if RA line) RA Reference authors Once or more (Optional if R line) RT Reference title Optional RL Reference location Once or more CC Comments or notes Optional DR Database cross-references Optional PE Protein existence Once KW Keywords Optional FT Feature table data Once or more in Swiss-Prot, optional in TrEMBL SQ Sequence header Once (blanks) Sequence data Once or more // Termination line Once; ends the entry

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FieldsinMoreDetail

●  IDline:IDEntryNameStatus;SequenceLength.

●  EntryName:upto11uppercasealphanumericcharactersX_Y-  Xisamnemoniccodeofatmost5alphanumericcharacters

-  Yisamnemonicspeciesiden)fica)oncodeofatmost5alphanumericcharacters

●  IDCYC_BOVINReviewed;104AA.

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●  ACline:ACAC_number_1;[AC_number_2;]...[AC_number_N;]

●  Accessionnumber:6or10characters1 2 3 4 5 6 7 8 9 10 [A-N,R-Z][0-9][A-Z] [A-Z,0-9][A-Z,0-9][0-9][O,P,Q] [0-9][A-Z,0-9][A-Z,0-9][A-Z,0-9][0-9][A-N,R-Z][0-9][A-Z] [A-Z,0-9][A-Z,0-9][0-9][A-Z] [A-Z,0-9] [A-Z,0-9] [0-9]

●  RegEx:[OPQ][0-9][A-Z0-9]{3}[0-9]|[A-NR-Z][0-9]([A-Z][A-Z0-9]{2}[0-9]){1,2}

●  Examples:P12345,Q1AAA9,A0A022YWF9

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●  DTline:date,DD-MMM-YYYY●  alwaysoneofthebiweeklyreleasedates

●  alwaysthreelines:-  dateofintegra)on-  dateofsequenceversion,sequenceversionX-  dateofentryversion,entryversionX

●  Example:DT01-FEB-1999,integratedintoUniProtKB/TrEMBL.DT15-OCT-2000,sequenceversion2.DT15-DEC-2004,entryversion5.

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●  DElines:-  threecategoriesandaddi)onalsubcategories-  containsarecommendedname-  besides:fullname,shortname,ECnumber-  alterna)venames:e.g.asanallergenorinbiotechnology,...

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DERecName:Full=AnnexinA5;DEShort=Annexin-5;DEAltName:Full=AnnexinV;DEAltName:Full=Lipocor)nV;DEAltName:Full=EndonexinII;DEAltName:Full=CalphobindinI;DEAltName:Full=CBP-I;DEAltName:Full=Placentalan)coagulantproteinI;DEShort=PAP-I;DEAltName:Full=PP4;DEAltName:Full=Thromboplas)ninhibitor;DEAltName:Full=Vascularan)coagulant-alpha;DEShort=VAC-alpha;DEAltName:Full=AnchorinCII;DERecName:Full=Granulocytecolony-s)mula)ngfactor;DEShort=G-CSF;DEAltName:Full=Pluripoie)n;DEAltName:Full=Filgras)m;DEAltName:Full=Lenogras)m;DEFlags:Precursor;

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●  OSline:origina)ngorganism●  OSHomosapiens(Human).●  OSRoussarcomavirus(strainSchmidt-RuppinA)(RSV-SRA)(Avianleukosis

OSvirus-RSA).

●  OClines:containthetaxonomicclassifica)onofthesourceorganismaccordingto(hMp://www.ncbi.nlm.nih.gov/Taxonomy/)

●  OCNode[;Node...].

●  OCEukaryota;Metazoa;Chordata;Craniata;Vertebrata;Euteleostomi;OCMammalia;Eutheria;Euarchontoglires;Primates;Catarrhini;Hominidae;OCHomo.

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RN,RP,RC,RX,RG,RA,RT,RL●  canoccurmul)ple)me●  orderinblockfixed

●  e.g:RN[1]RPNUCLEOTIDESEQUENCE[MRNA](ISOFORMSAANDC),FUNCTION,INTERACTIONRPWITHPKC-3,SUBCELLULARLOCATION,TISSUESPECIFICITY,DEVELOPMENTALRPSTAGE,ANDMUTAGENESISOFPHE-175ANDPHE-221.RCSTRAIN=BristolN2;RXPubMed=11134024;DOI=10.1074/jbc.M008990200;RAZhangL.,WuS.-L.,RubinC.S.;RT"AnoveladapterproteinemploysaphosphotyrosinebindingdomainandRTexcep)onallybasicN-terminaldomainstocaptureandlocalizeanRTatypicalproteinkinaseC:characteriza)onofCaenorhabdi)selegansRTCkinaseadapter1,aproteinthatavidlybindsproteinkinaseC3.“;RLJ.Biol.Chem.276:10463-10475(2001).

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CClines

●  freetext●  containsmostoftheannotatedinforma)on●  CC-!-TOPIC:Firstlineofacommentblock;

CCsecondandsubsequentlinesofacommentblock.

●  structuredbypredefinedtopicslike:Allergen,Alterna)veProducts,..,Cofactor,...,Disease,..Domain,...,Func)on,Interac)on,.......

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CC -!- ALLERGEN: Causes an allergic reaction in human. Minor allergen of!

CC bovine dander.!

CC -!- ALTERNATIVE PRODUCTS:!

CC Event=Alternative initiation; Named isoforms=2;!

CC Name=Alpha;!

CC IsoId=P51636-1; Sequence=Displayed;!

CC Name=Beta;!

CC IsoId=P51636-2; Sequence=VSP_018696;!

CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Peripheral!

CC membrane protein {ECO:0000250}. Secreted {ECO:0000250}. Note=The!

CC last 22 C-terminal amino acids may participate in cell membrane!

CC attachment.!

CC -!- SUBCELLULAR LOCATION: Isoform 2: Cytoplasm {ECO:0000305}.!

!

!

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CrossReferences

●  toomanytoenumerate●  extensivereferenceswithnucleo)dedatabases,e.g.:inEMBLFTCDS302..2674FT/protein_id="CAA03857.1“FT/db_xref="SWISS-PROT:P26345“FT/gene="recA“FT/product="RecAprotein“inSwiss=ProtDREMBL;AJ297977;CAC17465.1;-;Genomic_DNA.DREMBL;X56491;CAA39846.1;ALT_FRAME;mRNA.

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KeyWords/FeatureTable

●  KWKeyword[;Keyword...].●  helpstosearchresp.indexthedatabase

●  nolimits:KW3D-structure;Alterna)vesplicing;Alzheimerdisease;Amyloid;KWApoptosis;Celladhesion;Coatedpits;Copper;KWDirectproteinsequencing;Diseasemuta)on;Endocytosis;KWGlycoprotein;Heparin-binding;Iron;Membrane;Metal-binding;KWNotchsignalingpathway;Phosphoryla)on;Polymorphism;KWProteaseinhibitor;Proteoglycan;Serineproteaseinhibitor;Signal;KWTransmembrane;Zinc.

●  FeaturetablelikeGenBank/EMBL/DDBJ

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Programma)cAccess

●  hMp://www.uniprot.org/help/programma)c_access(rememberthislink!)

●  severalusecasesdocumented,butnotasanAPI●  bestway:usethewebinterfacetoconstruct/refineyourqueryfirstbeforeyoutrytoautomatetheprocess

BioinfRes SoSe 16

RetrievinganIndividualEntry

●  usessimpleURLwhichcanbebookmarked●  forindividualentries:hMp://www.uniprot.org/uniprot/P12345

●  defaultresultisawebpage

●  alterna)veformats:txt,xml,rdf,fasta,gff

●  specifiedviatheaccessionsuffix

●  structuredformatslikexmlorrdfcanincludereferencedentries

BioinfRes SoSe 16

UsingtheIDmappingservice

●  hMp://www.uniprot.org/help/programma)c_access#batch_retrieval_perl_example

●  useshMpPOSTmethod

●  convertsbetweendifferentdatabaseIDs

●  youhavetoknowthespecificabbrevia)onfortherespec)vedatabases

BioinfRes SoSe 16

RetrievingEntriesviaQueries

●  useshMpGETmethodi.e.●  thequerystringispartoftheURL

●  structuremightbequitecomplex

●  usethebrowsertoconfigurethequerystring●  moreseongareavailableviathequerybuilderhMp://www.uniprot.org/help/advanced_search

●  theURLlengthmightbelimitedto1000characters

BioinfRes SoSe 16

Examples●  hMp://www.uniprot.org/uniprot/P12345.txt●  hMp://www.uniprot.org/uniprot/P12345.xml

●  hMp://www.uniprot.org/uniref/UniRef90_P04259.xml

●  hMp://www.uniprot.org/uniref/UniRef90_P04259.rdf

●  hMp://www.uniprot.org/uniref/UniRef90_P04259.fasta

●  hMp://www.uniprot.org/uniref/UniRef90_P04259.tab