Post on 20-Apr-2020
Our funders:
Automation of Genetic Design
National Academy of Sciences
Industrialization of Biology Workshop
February 17, 2014
J. Christopher Anderson
Assistant Professor, Bioengineering
512E EBB, UC Berkeley
jcanderson@berkeley.edu
http://andersonlab.qb3.berkeley.edu
Synthetic
Biology
Today
Automated
Synthetic
Biology
synbiotrails.org Coming June 11th!
Act Ontology
Exhaustively compute the biosynthetically reachable space Enumerate all paths to each reachable chemical Rank those pathways for plausibility Provide linkage to the DNA sequences (parts) that encode each enzyme
True
The L0-L2 Reachable Space
Filtering the Reachable Space
Filtering the Reachable Space
Validation of an L2 target: Tylenol
Known Prediction Holes
Expression in foreign host Chemical modifications Multiple subunits Kinetic parameters Membrane/organellar localization Spontaneous reactions Volatile products
Big-Picture Bottlenecks
Intellectual property / freedom to operate Biosafety/biosecurity checks Regulatory analysis Target coordination Polyketides/Non-ribosomal peptides Polysaccharides Complex lipids Genetic circuits