Alyssa Hasty, PhDAssociate Professor

Molecular Physiology and Biophysics

Research, Scholarship or Creative Expression

Teaching Service

Research, Scholarship or Creative Expression

Teaching Service

National Reputation

CV (formatted according to their guidelines) Documentation of Teaching Form Critical References (description of your 5

most important papers and why they impacted the field)

Letters of Evaluation (minimum of 5) Letter from Chair/Chief of your department

https://medschool.vanderbilt.edu/faculty/details-appointments-and-promotions#supportdocs

A. MEDICAL SCHOOL COURSES. B. GRADUATE SCHOOL COURSES. C. CONTINUING EDUCATION. D. RESEARCH SUPERVISION. E. CLINICAL TEACHING. G. PRIMARY TEACHING ROLE (S).

Name Status Dates Did work result in publications?

1. Alli Nuotio-Antar PhD graduate student, Graduated April 2007

2004-2007 2 first author 1 co-author

2. Saraswathi Viswanathan Post-doctoral fellow 2004-2007 4 first author1 co-author

3. Kimberly R. Coenen PhD graduate student, Graduated March 2008

2004-2008 4 first author3 co-author

4. Bonnie K. Surmi PhD graduate student,Expected graduation 2009

2006-present

2 first author

5. Michelle R. Plummer Masters degree student (MLI),Graduated January 2007

2005-2007 1 first author4 co-author

6. Marnie L. Gruen Masters degree student (MLI),Graduated January 2007

2005-2007 3 first author5 co-author

7. Robin D. Atkinson IMSD student 2004-2006 1 first author2 co-author

8. Michael J. Puglisi Post-doctoral fellow 2008-present

1 co-author

9. Dario A. Gutierrez PhD graduate student 2008-present

1 first author1 co-author

10. Nick Khoo Post-doctoral fellow (visiting) 2008 Will result in at least 2 publications

The number differs by department

Please specify up to five references which represent the candidates most significant contributions. If the candidate if not first author, specify the role played by the candidate in the work described by these key publications.

“I have chosen the 5 most important and impactful papers out of 14 papers on which I am the senior corresponding author. These papers demonstrate the focus of my research program on mechanisms by which lipids induce macrophage inflammation. These inflammatory macrophages promote atherosclerotic lesion expansion when they are present in the arterial intima and insulin resistance when they are present in adipose tissue.”

How did they change our scientific knowledge?

Did they help you obtain grant funding? Did they help establish a collaboration? Did they bring you name recognition in

some other way?

Literature Citation #1. Names of all authors as listed sequentially on the publication: Title:  

Role of Candidate: I am the senior, corresponding author on this manuscript. The experiments were performed by Saraswathi Viswanathan, a post-doctoral fellow in my laboratory at that time. I was responsible for interpretation of the data. The manuscript was drafted by Dr. Viswanathan, and finalized and submitted by myself. 

This manuscript describes our observation that very low density lipoproteins (VLDL) induce inflammatory cytokine production in macrophages. In particular, we studied the time course and dose response expression of the chemokine, macrophage inflammatory protein-1 (MIP-1 ). Furthermore, we showed that the increase in inflammatory gene α αexpression coincided with the phosphorylation of the MAPK pathway members: ERK1/2, JNK, and p38 MAPK, and was ameliorated by U0126, an inhibitor of ERK1/2. Finally, inhibition of extracellular TG hydrolysis with Orlistat resulted in the absence of intracellular TG and FFA accumulation and was accompanied by the amelioration of ERK1/2 phosphorylation and MIP-1 gene expression. These results are important because αhypertriglyceridemia is a hallmark characteristic of obesity-related dyslipidemia, and macrophages play a critical role in both atherosclerotic lesion formation and insulin resistance. Thus, we concluded that elevations in TG-rich VLDL may contribute to the

pathogenesis of obesity by increasing inflammation in macrophages. These studies helped lay the foundation for both my American Diabetes Association grant to study MIP-1 in atherosclerosis and αinsulin resistance, as well as my NIH R01 to study the role of fatty acids in macrophage accumulation in adipose tissue.

These data have important implications to the pathogenesis of diet-induced obesity in humans, even when plasma lipid abnormalities are not present. This

publication was also instrumental in helping me obtain my R01. In addition, they brought me recognition in the field

of macrophage infiltration into AT, and I was subsequently asked to write two review articles on the topic.

Development of this mouse model has led to a collaborative effort between my laboratory and Drs. Judy Aschner, Jeff

Reese, and BC Paria to study the impact of maternal obesity on the cardiovascular outcomes in the offspring. We have

obtained P&F funding for this project and are currently obtaining preliminary data in order to submit a multi-PI NIH

R01 application next year. In addition, Dr. Coenen and I will be writing a review article on mouse models of the metabolic syndrome to be submitted to the journal Disease Models and Mechanisms this Fall.

This publication has lead to the development of 4 new collaborations for my lab. (1) We are collaborating with Dr.

Charles Wilkins at the University of Arkansas to assess phospholipid levels in tissues from these mice, and have a manuscript in submission.

(2) We are collaborating with Dr. Alan Tall at Columbia University to determine the effect of fish oil on the phagocytic index of arterial

macrophages. (3) We are collaborating with Dr. Edward Fisher at New York University to determine whether fish oil impacts apoB aggregation in liver. (4) We are collaborating with Dr. Naima Moustaid-Moussa at

the University of Tennessee Knoxville to determine whether fish oil impacts adipose tissue expression of genes involved in the renin

angiotensin system.

“Nationally recognized leaders in relevant area of scholarship”

Colleagues but NOT collaborators Someone in the same research group as a collaborator Someone who has followed your career and has an

interest in seeing you succeed Head of similar department as yours Could they say, “this person would certainly be given

T&P at my University” – be aware of other University T&P policies.

“The most frequent reason for deferral of Committee action is the need to request additional information from additional correspondents.”

Daniel Rader, MDUniversity of Pennsylvania School of Medicine Theodore Mazzone, MDProfessor of Medicine, University of Illinois at ChicagoChief, Section of Diabetes and Metabolism MC 797 Alan Daugherty, PhD, D.Sc.Professor of Medicine and PhysiologyGill Foundation Chair in Preventive CardiologyDirector, Cardiovascular Research Center 

Jay Heinecke, PhDProfessor of MedicineDivision of Metabolism, Endocrinology and Nutrition University of Washington School of Medicine Murray Huff, PhDProfessor, Departments of Medicine and BiochemistryUniversity of Western Ontario  Ira Tabas, MD. PhDProfessor of Medicine and Anatomy & Cell Biology, Department of MedicineColumbia University

https://medschool.vanderbilt.edu/faculty/suggested-format-curriculum-vitae

Include all sections, even if N/A Follow SOM guidelines carefully Within these guideline, make as aesthetically

pleasing as possible Don’t assume the reviewers will know

abbreviations that are common in your field There may be several months between when

your department T&P committee meet to when your package is sent to the University T&P committee –submit your most current CV

Name Education Licensure and Certification Academic Appointments Hospital Appointments Employment Professional Organizations Professional Activities Teaching Activities Other Activities Research Program Publications and Presentations (separate by

category)https://medschool.vanderbilt.edu/faculty/details-appointments-and-promotions#guidelines

Research, Scholarship or Creative Expression (National Recognition)

Because a senior investigator at another institution cares about your career

“Trade” invitations Invite yourself As part of collaboration Out of the blue

2002 – 1 2003 – 1 2006 – 3 2007 – 5 2008 – 5 2009 - 2

Only 4 of 17 were from people I did

not know

Alan Daugherty Invitation to speak at UK Invitation to speak at ATVB Invitation to speak at Gordon Conference Invitation to be on ATVB planning committee

2003-2007 American Heart Association Southeast/OhioValley Chapter Grant Committee (met Carole Stzalryd)

We traded invitations 12/04/08 University of Maryland. Clinical

Nutrition Research Unit Seminar.

2003-2007 American Heart Association Southeast/OhioValley Chapter Grant Committee (met Suzanne Mohr)

12/10/07 Case Western Reserve University - Diabetes Research Seminar.

Met Pete Bodary at ATVB conference in 2006

We traded invitations 04/17/07 Wayne State University -

Nutrition and Food Sciences Seminar Series.

Met Heddwen Brooks at a Women’s Faculty Leadership conference

We traded invitations 10/13/06 University of Arizona – Diabetes

Seminar Series.

Met Alan Chait who spoke in DRTC seminar series

I initiated a collaboration and “invited myself” to visit with him to discuss

11/15/07 University of Washington - Endocrinology Research Conference.

Met Jay Heinecke at U Wash and again at a Gordon Conference

Met Martin Meyers who spoke in MPB seminar series

10/11/07 University of Michigan - Division of Metabolism, Endocrinology, and Diabetes Seminar Series.

He won an award at the Obesity Conference last year and invited me to a party. I met Susan Fried there who is the current chair of the Obesity Society and asked me to speak at their next annual meeting.

Research, Scholarship or Creative Expression (National Recognition)

1. Articles in refereed journals: List chronologically

2. Books, book chapters, invited review articles

3. Letters to editor, book reviews, editorials (optional)

4. Abstracts (optional)

5. Presentations at Scientific Meetings

Separate by category Doesn’t say you can’t include “In Submission”

or “In Revision” I would not include “In Preparation”

Total 56 original 2 reviews 1 book chapter

23 were first author or from my lab

From 2003 27 original 2 reviews 1 book chapter

17 were from my lab

Research, Scholarship or Creative Expression (National Recognition)

Previous P&Fs and Junior Faculty Grants ADA Career Development Award R01 Choice of Study Section and NIH Institute is

VERY important

Found an exceptional lab manager who has been with me since day 1

Spent my development funds to hire people so that I published fairly early

Wrote for and obtained junior faculty grants early My development funds lasted longer Showed evidence of obtaining funding

Department paid to have my R01 read by outside person

Used my mentoring committee for advice

The wrong person is not better than no person.

Even while working on projects you have funded, look toward the future of where your field will be going next.

If you are moving away from a certain direction, finish and publish those studies as quickly as possible.

Do NOT avoid conflict – especially if it is with someone in your lab group.

Find out who is on the T&P committee and talk to them about your CV

Set up a meeting with Dr. Penn or Dr. Raiford to talk about your CV

You NEED someone on your side in your department for when you are discussed!

Reserve time on your calendar to read/write Pay it forward.