Post on 31-May-2020
©2012 Waters Corporation 1
ACQUITY Systems with 2D Technology
MS Technology Day
Sesión Alimentación y Medio Ambiente
Barcelona, 21 de marzo de 2012
©2012 Waters Corporation 2
Why Bother with 2D Chromatography?
©2012 Waters Corporation 3
Two Dimensional Chromatography Principal
2.1
2.2
Separation Method #1
1
2
Separation Method #2
1.1
1.2
©2012 Waters Corporation 4
ACQUITY UPLC Systems with 2D Technology Solves Four Key Customer Challenges
Increase Selectivity & Sensitivity
Eliminate Unwanted
Interferences
Mobile Phase Flexibility
Characterize the Most Complex Samples
©2012 Waters Corporation 5
Agenda
Waters Two Dimensional UPLC Capability
Two Dimensional Functions and Analytical Benefits — Trapping
— Heart Cutting
— Parallel Column Regeneration
— At-Column Dilution
©2012 Waters Corporation 6
Simple to Use Easy to Implement Proven UPLC benefits
—Resolution —Sensitivity —Productivity
Compatible with a wide range of Chemistries
ACQUITY Systems with 2D Technology
©2012 Waters Corporation 7
ACQUITY UPLC Column Manager Designed for 2D Operation
Two Easy-to-access, 6-port 2-position valves Two independent temperature controlled zones Low Volume Active Pre-Heaters Ensure best
Chromatographic performance run to run and system to system
©2012 Waters Corporation 8
ACQUITY Systems with 2D Technology Software Control through MassLynx
Binary Solvent Manager Quaternary Solvent Manager
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ACQUITY Systems with 2D Technology Software Control through MassLynx
Simple Control of Valves by Timed Events in Column Manager
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Trapping
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Time 3.60 3.80 4.00 4.20 4.40 4.60 4.80 5.00 5.20 5.40
%
0
100
MRM of 6 Channels ES+ 327 > 269.9
1.46e6 4.31
250 uL Clozapine 1 ppb 2D – 3 min gradient
250 uL Clozapine 1 ppb 1D – 3 min gradient
Peak distortion Volume Overload
20 uL Clozapine 1 ppb 1D – 3 min gradient
Increase Sample Loading and Sensitivity with Trap and Transfer Configuration
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Heart Cutting
©2012 Waters Corporation 17
ACQUITY UPLC and Xevo TQ MS Bioanalysis
1D Separation
©2012 Waters Corporation 18
Running 1D
1
BSM 1 +SM
BSM 2
Waste
1D column
BSM2
2D column
Trapping Column
©2012 Waters Corporation 21
Heart Cutting– ACQUITY UPLC with 2D Technology and Xevo TQ MS
1D Separation
2D Separation
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ACQUITY UPLC with 2D Technology – Impurity Profiling Heart Cut
Heart Cut
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2D Heart-cutting Benefits
Orthogonality
— In mathematics, two vectors are orthogonal if they are perpendicular, i.e., they form a right angle. The word comes from the Greek ὀρθός (orthos), meaning "straight", and γωνία (gonia), meaning "angle". For example, a subway and the street above, although they do not physically intersect, are orthogonal if they cross at a right angle.
Specificity of a method
Enhanced sensitivity in MS by dramatic reduction of matrix effect.
Possibility to load more and refocused the 1D into the 2D
©2012 Waters Corporation 24
1D Chromatogram
Connect Waste tubing to MS
Leave the valve in position 1 to see the 1D peak eluting
This is the 1D peak we will have to trap
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Separation is performed on the 2D column
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2D on the Xevo TQ-S 5pg/ml Plasma Estradiol
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1D vs 2D
1D
2D
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Parallel Column Regeneration
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Parallel Column Regeneration
Increase Overall Speed of Analysis — Analytical Separation Performed while 2nd Column is Regenerated
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Parallel Column Regeneration Case Study
Complex Samples Requiring Long Run times of 35 minutes
Regeneration and Requilibration requiring 15 minutes
24 Hour Operation Samples Run 1 Dimensional ACQUITY UPLC 28 ACQUITY UPLC with 2D Technology 41
30% Increase in Throughput
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Summary – ACQUITY Systems with 2D Technology
Simple to Use
Easy to Implement
Straightforward to operate in various 2D functions — Trapping
— Heart Cutting
— Parallel Column Regeneration
— At-Column Dilution