Bradley W. Christian, McLennan Community College© 2016 Pearson Education, Inc.

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Chapter 6: Microbial Growth

Physical Requirements

ChemicalRequirements

Growth of Bacterial Cultures

Measurement of GrowthCulture Media

FINAL ROUND

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Topic 1: Physical Requirements $100 Question

The optimum pH for MOST bacteria is near

a. 1.b. 5.c. 7.d. 9.

ANSWER

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Topic 1: Physical Requirements $100 Answer

The optimum pH for MOST bacteria is near

a. 1.b. 5.c. 7.d. 9.

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Topic 1: Physical Requirements $200 Question

A psychrophile has an optimal growth temperature of about

a. 15°C.b. 25°C.c. 37°C.d. 100°C.

ANSWER

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Topic 1: Physical Requirements $200 Answer

A psychrophile has an optimal growth temperature of about

a. 15°C.b. 25°C.c. 37°C.d. 100°C.

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Topic 1: Physical Requirements $300 Question

Organisms that require high salt concentrations for growth are called

a. thermophiles.b. obligate halophiles.c. acidophiles.d. anaerobes.

ANSWER

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Topic 1: Physical Requirements $300 Answer

Organisms that require high salt concentrations for growth are called

a. thermophiles.b. obligate halophiles.c. acidophiles.d. anaerobes.

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Topic 1: Physical Requirements $400 Question

What is/are the energy source(s) utilized by primary producers in the hydrothermal vents on the ocean floor?

a. lightb. hydrogen sulfidec. organic moleculesd. carbon dioxide

ANSWER

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Topic 1: Physical Requirements $400 Answer

What is/are the energy source(s) utilized by primary producers in the hydrothermal vents on the ocean floor?

a. lightb. hydrogen sulfidec. organic moleculesd. carbon dioxide

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Topic 1: Physical Requirements $500 Question

Addition of salts preserves foods because they

a. lower pH.b. increase osmotic pressure.c. create an isotonic environment.d. lower osmotic pressure.

ANSWER

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Topic 1: Physical Requirements $500 Answer

Addition of salts preserves foods because they

a. lower pH.b. increase osmotic pressure.c. create an isotonic environment.d. lower osmotic pressure.

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Topic 2: Chemical Requirements $100 Question

Chemoautotrophs and photoautotrophs derive their carbon from

a. carbon dioxide.b. proteins.c. lipids.d. carbohydrates.

ANSWER

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Topic 2: Chemical Requirements $100 Answer

Chemoautotrophs and photoautotrophs derive their carbon from

a. carbon dioxide.b. proteins.c. lipids.d. carbohydrates.

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Topic 2: Chemical Requirements $200 Question

Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called

a. denitrification.b. photosynthesis.c. nitrogen fixation.d. nitrogen synthesis.

ANSWER

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Topic 2: Chemical Requirements $200 Answer

Some microorganisms use gaseous nitrogen directly from the atmosphere in a process called

a. denitrification.b. photosynthesis.c. nitrogen fixation.d. nitrogen synthesis.

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Topic 2: Chemical Requirements $300 Question

An organism that uses oxygen when present but can grow without oxygen is called a(n)

a. obligate aerobe.b. facultative anaerobe.c. microaerophile.d. aerotolerant anaerobe.

ANSWER

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Topic 2: Chemical Requirements $300 Answer

An organism that uses oxygen when present but can grow without oxygen is called a(n)

a. obligate aerobe.b. facultative anaerobe.c. microaerophile.d. aerotolerant anaerobe.

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Topic 2: Chemical Requirements $400 Question

Which organism is killed by atmospheric oxygen?

a. facultative anaerobeb. obligate aerobec. obligate anaerobed. aerotolerant anaerobe

ANSWER

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Topic 2: Chemical Requirements $400 Answer

Which organism is killed by atmospheric oxygen?

a. facultative anaerobeb. obligate aerobec. obligate anaerobed. aerotolerant anaerobe

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Topic 2: Chemical Requirements $500 Question

Toxic hydrogen peroxide in some bacteria can be broken down into water and oxygen by the enzyme

a. superoxide dismutase.b. peroxidase.c. hydroxylase.d. catalase.

ANSWER

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Topic 2: Chemical Requirements $500 Answer

Toxic hydrogen peroxide in some bacteria can be broken down into water and oxygen by the enzyme

a. superoxide dismutase.b. peroxidase.c. hydroxylase.d. catalase.

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Topic 3: Growth of Bacterial Cultures $100 Question

Generation time can best be defined as

a. the length of time it takes for lag phase.b. the duration of log phase.c. the time it takes for a cell to divide.d. how many generations a cell can divide.

ANSWER

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Topic 3: Growth of Bacterial Cultures $100 Answer

Generation time can best be defined as

a. the length of time it takes for lag phase.b. the duration of log phase.c. the time it takes for a cell to divide.d. how many generations a cell can divide.

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Topic 3: Growth of Bacterial Cultures $200 Question

Bacterial growth is usually graphed using ________ scales.

a. arithmeticb. exponentialc. logarithmic (semi-logarithmic)d. inverse

ANSWER

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Topic 3: Growth of Bacterial Cultures $200 Answer

Bacterial growth is usually graphed using ________ scales.

a. arithmeticb. exponentialc. logarithmic (semi-logarithmic)d. inverse

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Topic 3: Growth of Bacterial Cultures $300 Question

In which growth phase is there intense activity preparing for population growth, but no increase in population?

a. stationary phaseb. log phasec. death phased. lag phase

ANSWER

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Topic 3: Growth of Bacterial Cultures $300 Answer

In which growth phase is there intense activity preparing for population growth, but no increase in population?

a. stationary phaseb. log phasec. death phased. lag phase

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Topic 3: Growth of Bacterial Cultures $400 Question

Which of the following is NOT a possible reason why exponential growth stops?

a. accumulation of waste productsb. exhaustion of nutrientsc. harmful changes in pHd. bacteria becoming dormant

ANSWER

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Topic 3: Growth of Bacterial Cultures $400 Answer

Which of the following is NOT a possible reason why exponential growth stops?

a. accumulation of waste productsb. exhaustion of nutrientsc. harmful changes in pHd. bacteria becoming dormant

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Topic 3: Growth of Bacterial Cultures $500 Question

In the stationary phase,

a. the number of microbial deaths exceeds the number of new cells formed.

b. the number of microbial deaths is equal to the number of new cells formed.

c. new cells are no longer formed because of adverse environmental conditions.

d. cellular reproduction is most active.ANSWER

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Topic 3: Growth of Bacterial Cultures $500 Answer

In the stationary phase,

a. the number of microbial deaths exceeds the number of new cells formed.

b. the number of microbial deaths is equal to the number of new cells formed.

c. new cells are no longer formed because of adverse environmental conditions.

d. cellular reproduction is most active.

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Topic 4: Culture Media $100 Question

Which type of medium suppresses the growth of unwanted bacteria and encourages growth of desired microbes?

a. differential mediab. complex mediac. selective mediad. enrichment media

ANSWER

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Topic 4: Culture Media $100 Answer

Which type of medium suppresses the growth of unwanted bacteria and encourages growth of desired microbes?

a. differential mediab. complex mediac. selective mediad. enrichment media

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Topic 4: Culture Media $200 Question

Microbes that are introduced into a culture medium to initiate growth are called a(n)

a. inoculum.b. culture.c. specimen.d. medium.

ANSWER

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Topic 4: Culture Media $200 Answer

Microbes that are introduced into a culture medium to initiate growth are called a(n)

a. inoculum.b. culture.c. specimen.d. medium.

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Topic 4: Culture Media $300 Question

What is added to a medium when it is desirable to grow bacteria on a solid medium?

a. glucoseb. magnesium sulfatec. sodium thioglycollated. agar

ANSWER

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Topic 4: Culture Media $300 Answer

What is added to a medium when it is desirable to grow bacteria on a solid medium?

a. glucoseb. magnesium sulfatec. sodium thioglycollated. agar

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Topic 4: Culture Media $400 Question

A culture medium made of extracts from yeasts, meat, or plants is a ________ medium.

a. complexb. selectivec. differentiald. chemically defined

ANSWER

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Topic 4: Culture Media $400 Answer

A culture medium made of extracts from yeasts, meat, or plants is a ________ medium.

a. complexb. selectivec. differentiald. chemically defined

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Topic 4: Culture Media $500 Question

Labs intended for highly infectious airborne pathogens, such as tuberculosis, are classified as

a. BSL-1.b. BSL-2.c. BSL-3.d. BSL-4.

ANSWER

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Topic 4: Culture Media $500 Answer

Labs intended for highly infectious airborne pathogens, such as tuberculosis, are classified as

a. BSL-1.b. BSL-2.c. BSL-3.d. BSL-4.

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Topic 5: Measurement of Growth $100 Question

A spectrophotometer can be used to measure

a. metabolic activity.b. turbidity.c. dry weight.d. pathogenicity.

ANSWER

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Topic 5: Measurement of Growth $100 Answer

A spectrophotometer can be used to measure

a. metabolic activity.b. turbidity.c. dry weight.d. pathogenicity.

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Topic 5: Measurement of Growth $200 Question

The method in which a measured volume of bacterial suspension is placed within a defined area on a microscope slide is called the

a. direct microscope count.b. pour plate method.c. spread plate method.d. most probable number method.

ANSWER

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Topic 5: Measurement of Growth $200 Answer

The method in which a measured volume of bacterial suspension is placed within a defined area on a microscope slide is called the

a. direct microscope count.b. pour plate method.c. spread plate method.d. most probable number method.

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Topic 5: Measurement of Growth $300 Question

Which process is used to ensure that plates contain 30 to 300 colonies when counted?

a. turbidityb. serial dilutionc. filtrationd. pour plates

ANSWER

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Topic 5: Measurement of Growth $300 Answer

Which process is used to ensure that plates contain 30 to 300 colonies when counted?

a. turbidityb. serial dilutionc. filtrationd. pour plates

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Topic 5: Measurement of Growth $400 Question

Which is the best technique to use to measure the growth of filamentous organisms?

a. most probable number methodb. direct microscopic countc. turbidityd. dry weight

ANSWER

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Topic 5: Measurement of Growth $400 Answer

Which is the best technique to use to measure the growth of filamentous organisms?

a. most probable number methodb. direct microscopic countc. turbidityd. dry weight

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Topic 5: Measurement of Growth $500 Question

Which method is a statistical estimating technique used to determine the number of bacteria in a sample?

a. turbidityb. spread plate methodc. most probable number methodd. pour plate method

ANSWER

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Topic 5: Measurement of Growth $500 Answer

Which method is a statistical estimating technique used to determine the number of bacteria in a sample?

a. turbidityb. spread plate methodc. most probable number methodd. pour plate method

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FINAL ROUND Question

Starting with one bacterial cell, how many cells would you have after 10 generations?

a. 10b. 20c. 200d. 1024

ANSWER

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FINAL ROUND Answer

Starting with one bacterial cell, how many cells would you have after 10 generations?

a. 10b. 20c. 200d. 1024

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