0 Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved...

Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved substances.Chromatography can be used to identify drugs, poisons and many other substances.Separation is determined by the molecular size and/or charge

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All forms of chromatography involve 2 phases The Two Phases1. stationary (absorbent) phase the material on which the separation takes place2. mobile phase - the solvent transports the sampleTypes of Chromatography - 5 of them


Gas ChromatographyGas Chromatographya gas separates the componentsthey are then ionized and an electrical signal is recordedmobile phase - carrier gasstationary phase - thin film of liquid


HPLCHigh Performance Liquid Chromatography (HPLC)- done at room temp. - advantageous for chemicals that are heat sensitive or volatile - example LSD- a liquid (mobile phase) is pumped through a column containing fine particles (stationary phase)


TLCThin - Layer Chromatography (TLC)-A plate is coated with a granular gel - usually silica gel or aluminum oxide (stationary phase)- the substance to be separated is carried up the plate by capillary action - the substance with most affinity for the plate will rise the farthest.4. Paper chromatographySame as TLC but paperis used as stationary phase


ElectrophoresisElectrophoresis - Similar to TLC but the substance is separated through a gel by electric current- Due to different size and charge substances will move across the plase at different speeds.


Paper Chromatography of InkTwo samples of black ink from two different manufacturers have been characterized using paper chromatography.


Retention Factor (Rf)A number that represents how far a compound travels in a particular solventIt is determined by measuring the distance the compound traveled and dividing it by the distance the solvent traveled.


Solve for Rf


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0 Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved...

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Transcript of 0 Chromatography is a method of physically separating mixtures of gases, liquids, or dissolved...

From: poster … is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the mixture or

Experiment #2: - North Seattle Collegefacweb.northseattle.edu/aburns/C162/121NLab3bTLC.docx · Web viewOne of the most modern methods of separating mixtures in chemistry is chromatography.

Liquid chromatography - ftp.mn-net.com · 144 Basics High performance liquid chromatography (HPLC) is part of liquid chromatographic separating processes of substance mixtures

What is Chromatography? Chromatography is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify.

Separation Methods · Separating Mixtures Substances in a mixture are physically combined, so processes bases on differences in physical properties are used to separate component

Gas Chromatography 427 PHC. Gas Chromatograph Gas chromatography (GC), is a common type of chromatography used in analytic chemistry for separating and.

Chromatography is a method for separating the components of a mixture by differential adsorption between a stationary phase and a mobile (moving) phase.

Separating Ferrocene and Acetylferrocene by adsorption column chromatography

Paper Chromatography and Separating immiscible liquids

Separating plant pigments in Papaver somniferum using ...biotapp.weebly.com/uploads/2/5/3/1/2531617/plant_pigments.pdf · Separating plant pigments in Papaver somniferum using Chromatography

Separation Techniques Methods of Separating Mixtures Magnet Filter Decant Evaporation Centrifuge Chromatography Distillation.

Paper Chromatography: Separating and Identifying Metal Ions

1 Froth Flotation – Fundamental · PDF file1 1 Froth Flotation – Fundamental Principles Froth flotation is a highly versatile method for physically separating particles based on

Chapter 21 Principles of Chromatography. Chromatography is the most powerful tool for separating & measuring the components of a complex mixture. Quantitative.

Chromatography Separating different pigment solutes from food coloring.

Security Domain Libraries Engineering Guide · to, separating networks, physically protecting system components, user awareness programs, in-depth security, and so on. For additional

Acclaim HILIC-10 Columns - Thermo Fisher Scientific...Hydrophilic Interaction Liquid Chromatography) column for separating highly hydrophilic analytes. It is based on high-purity,

CHROMATOGRAPHY · Adsorption chromatography Ion exchange chromatography Partition chromatography Gel (size exclusion) chromatography Affinity chromatography Capillary chromatography

Gas Chromatography. Gas Chromatograph Gas chromatography (GC), is a common type of chromatography used in analytical chemistry for separating and analyzing.

Separating mixtures and solutions: Chromatography Mixtures and Solutions.