© 2007 MSA

MSA BIOSENSOR™MSA BIOSENSOR™

Biological Agent Biological Agent DetectorDetector

© 2007 MSA

ProblemProblem

Major Bio-Threats Anthrax Ricin SEB (staph infection) Botulism Plague Cholera Tularemia Smallpox

© 2007 MSA

Homeland DefenseHomeland Defense

Personnel responsible: First Responders Fire Departments Police Departments HazMat teams Hospital Triage Military F.B.I.

© 2007 MSA

SolutionSolution

Needs for Homeland Defense: On-site/portable analysis Rapid detection Easy to use Excellent sensitivity Low false positives Test multiple targets at same time

© 2007 MSA

Handheld Detector forBiological Agents

Handheld Detector forBiological Agents

MSA BIOSENSOR™MSA BIOSENSOR™Biological Agent DetectorBiological Agent Detector

© 2007 MSA

Product PerformanceProduct Performance

B. Anthracis sensitivity: <10,000 spores

Ricin sensitivity:

0.5 ngFalse positives:

< 1 in 1,000,000Time to answer:

Results in 5 minutes

© 2007 MSA

Product PerformanceProduct PerformanceThe MSA BIOSENSOR 2200R Biological Agent Detector has an established track record in real-world applications and has been subject to successful third-party testing.

© 2007 MSA

MSA BIOSENSOR 2200R DetectorMSA BIOSENSOR 2200R Detector Handheld Dimensions

11”(L) x 10”(W) x 5”(H) Weight: 7 pounds Battery life: 50 + tests LCD screen 4 button user interface Integrated RFID Data storage for up to 50 test results Serial port for data download Training time: 1 hour

© 2007 MSA

CartridgeCartridge

Single-useSelf-sealingReagents in cartridgeShelf life:12 monthsStorage conditions: room temperatureRFID for automatic cartridge identification

Available Targets•Anthrax •Ricin•SEB (staph infection)

© 2007 MSA

CartridgeCartridge

Detection Chamber

© 2007 MSA

CartridgeCartridge

Mixing Ball

© 2007 MSA

CartridgeCartridge

Magnetic Microsphere with specific Biological antibodies (Mag)

© 2007 MSA

CartridgeCartridge

Fluorescent Tag with specific Biological antibodies (Tag)

© 2007 MSA

Ease of UseEase of Use Operation

1. Insert wash syringe into cartridge

2. Collect sample and draw into sample syringe

3. Insert sample syringe into cartridge, push on plunger

4. Shake cartridge for one minute

5. Insert into instrument

6. Wait 3 minutes, then push down on wash syringe

7. Push “READ” button, obtain easy-to-interpret results:

RED LIGHT – TARGET PRESENT

GREEN LIGHT – NO TARGET PRESENT

© 2007 MSA

BIOSENSOR Assay FormatBIOSENSOR Assay Format

Dynamic surface generation and detection Combines benefits of free solution and lateral flow type assays Concentration increases sensitivity Wash step improves assay robustness Compatible with continuous monitor system

MIX BIND/MAGNETIZE

WASH READ

© 2007 MSA

BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology

1. MIX Sample is mixed with sensing solution: Magnetic microspheres and fluorescent tag.

© 2007 MSA

BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology

2. BIND Sensing materials bind spores during

mixing and incubation.

© 2007 MSA

BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology

3. MAGNETIZE All bound and unbound magnetic

material separate to surface.

© 2007 MSA

BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology

4.WASH Unbound TAG remains in solution

and is washed away.

© 2007 MSA

BIOSENSOR Assay TechnologyBIOSENSOR Assay Technology

5. READ Concentrated sample (pellet) is illuminated

and emits a signal if target is present.

© 2007 MSA

< 10,000 spores easily detected within five minutes.

Anthrax (bacillus anthracis) Limit of DetectionAnthrax (bacillus anthracis) Limit of Detection

© 2007 MSA

Ricin (ricinus communis) Limit of DetectionRicin (ricinus communis) Limit of Detection

< 1 ng detected within five minutes.

© 2007 MSA

Anthrax/Ricin Multiplex Limit of DetectionAnthrax/Ricin Multiplex Limit of Detection

< 10,000 BA spores easily detected within 5 minutes.

< .5 Ricin ng detected within 5 minutes.

© 2007 MSA

Anthrax (bacillus anthracis) Interference StudyAnthrax (bacillus anthracis) Interference Study

No false positives from common interferents

© 2007 MSA

Anthrax Spores Interference Study Anthrax Spores Interference Study 0.1 mg/mL of the indicated powders were mixed with 5000 Pasteur spores and analyzed on the Anthrax specific cartridges.

Added Powder (0.1mg/mL) Response (mV) Result

None (neat Pasteur spike) 312 - 573 True Positive

Spackling Powder 179 True Positive

Baking Soda (Arm & Hammer) 934 True Positive

Instant Non-fat Dried Milk (Nestle Carnation) 652 True Positive

Talcum Powder (Equate) 453 True Positive

Flour (Gold Medal) 499 True Positive

Salt (Morton) 550 True Positive

Yeast (Red Star) 609 True Positive

Powdered Sugar (Confectioners) 294 True Positive

Dipel™ (Abbot Laboratories) 238 True Positive

Chalk (Bison) 272 True Positive

Foot Powder (Total Body) 522 True Positive

Ajax™ with Bleach (Colgate-Palmolive) 213 True Positive

Non-fat Dairy Creamer (Great Value) 520 True Positive

Kaolin (Fisher-Scientific) 528 True Positive

Bentonite (Aldrich) 356 True Positive

Aerosil R812S™ (Degussa) 485 True Positive

© 2007 MSA

Ricin (A Chain) Assay Interference Study Ricin (A Chain) Assay Interference Study

No false positives from common interferents.

0

50

100

150

200

250

300

RF

U

Ricin Assay Interference StudyInterferent concentration = 100 ug/mL

© 2007 MSA

Other Bacillus Spores Interference Study Other Bacillus Spores Interference Study

No false positives from nearest neighbor spores.

© 2007 MSA

MSA BIOSENSOR 2200R Detector PlatformMSA BIOSENSOR 2200R Detector Platform

On-site/portable analysis Rapid detection Excellent sensitivity Extremely easy to use Excellent protection against false positives Single test capabilities for multiple bio-threats

(anthrax/ricin combo)

© 2007 MSA

Target AvailabilityTarget Availability Available Targets

Anthrax/ricin multi-agentAnthrax Ricin SEB (staph infection)

Under DevelopmentBotulinumPlagueCholeraTularemiaSmallpoxWest Nile virusOther agents in

development

© 2007 MSA

BIOSENSOR 2200R Biological Agent DetectorCompetitive Comparison

BIOSENSOR 2200R Biological Agent DetectorCompetitive Comparison

© 2007 MSA

Ordering InformationOrdering Information MSA BIOSENSOR 2200R Detector Kit (10084834) Includes:

● BIOSENSOR 2200R Detector ● Cartridge starter kit:

-3 Anthrax/ricin combo

-3 Positive control-1 Anthrax-1 Ricin-2 Negative control

● Battery charger ● Instruction manual ● Laminated quick instruction guide ● Carrying bag for all contents

© 2007 MSA

Cartridge Replacement PlansCartridge Replacement Plans

Plans based upon expected annual white powder calls

Low (6 per year) - 10084753 Medium (2 per month) - 10084754 High (5 per month) - 10084755

© 2007 MSA

Cartridge Replacement PlansCartridge Replacement Plans

Low (1 every 2 months) Includes:

6 Anthrax/Ricin Combo 6 Positive Control 2 Anthrax 2 Ricin 2 Negative Control 2 Wet sample kits 2 Calibration kits

Includes 2 positive controls

All biohazard targets kits include dry sample kit

© 2007 MSA

Cartridge Replacement PlansCartridge Replacement Plans

Medium (2 per month) Includes:

24 Anthrax/Ricin Combo 24 Positive Control 6 Anthrax 6 Ricin 6 Negative Control 4 Wet sample kits 2 Calibration kits

Includes 2 positive controls

All biohazard targets kits include dry sample kit

© 2007 MSA

Cartridge Replacement PlansCartridge Replacement Plans

High (5 per month) Includes:

60 Anthrax/Ricin Combo 60 Positive Control 8 Anthrax 8 Ricin 8 Negative Control 6 Wet sample kits 2 Calibration kits

Includes 2 positive controls

All biohazard targets kits include dry sample kit

© 2007 MSA

MSA BIOSENSOR™MSA BIOSENSOR™Biological Agent DetectorBiological Agent Detector